Effects of ethyl acetate extract of Salsola collina on brain-gut peptides and interstitial cells of gastric Cajal in rats with diabetic gastroparesis
| Title: | Effects of ethyl acetate extract of Salsola collina on brain-gut peptides and interstitial cells of gastric Cajal in rats with diabetic gastroparesis |
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| Authors: | Xinling Zhao, Hongbo Wang, Ziwei Zhang, Hong Jin, Yanling Gong |
| Source: | Iranian Journal of Basic Medical Sciences, Vol 23, Iss 9, Pp 1218-1224 (2020) |
| Publisher Information: | Mashhad University of Medical Sciences, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Medicine |
| Subject Terms: | brain, gut peptides cajal interstitial cells diabetic gastroparesis ethyl acetate extract gastric motility salsola collina, Medicine |
| More Details: | Objective(s): Effects of ethyl acetate extract of Salsola collina (EES) on brain-gut peptides and interstitial cells of gastric Cajal in rats with diabetic gastroparesis were explored. Materials and Methods: Rats were divided into six groups: normal control group (NC), diabetic gastroparesis model group (DGP), low, medium, and high dose of EES groups (LES, MES, and HES, respectively), and metoclopramide positive group (MPG). DGP rats were induced by streptozotocin (STZ) combined with a high-sugar-high-fat diet. The gastric emptying was measured by the phenol red labeling method. Enzyme-linked immunosorbent assay (ELISA) was employed to determine the concentrations of serum ghrelin, gastrin (GAS), somatostatin (SS), and vasoactive intestinal peptide (VIP). The expressions of c-Kit and its natural ligand stem cell factor (SCF) in gastric tissues were determined by Western blot and immunofluorescence. Results: Gastric emptying rate increased in a different degree after intervention by EES, among which MES and HES groups showed a significant effect (compared with DGP, PConclusion: EES significantly improved gastric emptying by regulating gastrointestinal hormone excretion and c-Kit/SCF signaling pathway. Our study provides a pharmacological basis for the use of the EES in the treatment of DGP. However, the detailed molecular mechanism remains to be clarified. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2008-3866 2008-3874 |
| Relation: | http://ijbms.mums.ac.ir/article_16171_58f2faef52b2baf5c3131d7e9ad9974c.pdf; https://doaj.org/toc/2008-3866; https://doaj.org/toc/2008-3874 |
| DOI: | 10.22038/ijbms.2020.43521.10223 |
| Access URL: | https://doaj.org/article/399a4b57b1494d61814ff61a3e5ad192 |
| Accession Number: | edsdoj.399a4b57b1494d61814ff61a3e5ad192 |
| Database: | Directory of Open Access Journals |
| ISSN: | 20083866 20083874 |
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| DOI: | 10.22038/ijbms.2020.43521.10223 |
| Published in: | Iranian Journal of Basic Medical Sciences |
| Language: | English |