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Longitudinal analysis within one hospital in sub-Saharan Africa over 20 years reveals repeated replacements of dominant clones of Klebsiella pneumoniae and stresses the importance to include temporal patterns for vaccine design considerations

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Title: Longitudinal analysis within one hospital in sub-Saharan Africa over 20 years reveals repeated replacements of dominant clones of Klebsiella pneumoniae and stresses the importance to include temporal patterns for vaccine design considerations
Authors: Eva Heinz, Oliver Pearse, Allan Zuza, Sithembile Bilima, Chisomo Msefula, Patrick Musicha, Patriciah Siyabu, Edith Tewesa, Fabrice E. Graf, Rebecca Lester, Samantha Lissauer, Jennifer Cornick, Joseph M. Lewis, Kondwani Kawaza, Nicholas R. Thomson, Nicholas A. Feasey
Source: Genome Medicine, Vol 16, Iss 1, Pp 1-17 (2024)
Publisher Information: BMC, 2024.
Publication Year: 2024
Collection: LCC:Medicine
LCC:Genetics
Subject Terms: AMR, Malawi, Genome surveillance, Healthcare-associated, Nosocomial infections, Surface antigens, Medicine, Genetics, QH426-470
More Details: Abstract Background Infections caused by multidrug-resistant gram-negative bacteria present a severe threat to global public health. The WHO defines drug-resistant Klebsiella pneumoniae as a priority pathogen for which alternative treatments are needed given the limited treatment options and the rapid acquisition of novel resistance mechanisms by this species. Longitudinal descriptions of genomic epidemiology of Klebsiella pneumoniae can inform management strategies but data from sub-Saharan Africa are lacking. Methods We present a longitudinal analysis of all invasive K. pneumoniae isolates from a single hospital in Blantyre, Malawi, southern Africa, from 1998 to 2020, combining clinical data with genome sequence analysis of the isolates. Results We show that after a dramatic increase in the number of infections from 2016 K. pneumoniae becomes hyperendemic, driven by an increase in neonatal infections. Genomic data show repeated waves of clonal expansion of different, often ward-restricted, lineages, suggestive of hospital-associated transmission. We describe temporal trends in resistance and surface antigens, of relevance for vaccine development. Conclusions Our data highlight a clear need for new interventions to prevent rather than treat K. pneumoniae infections in our setting. Whilst one option may be a vaccine, the majority of cases could be avoided by an increased focus on and investment in infection prevention and control measures, which would reduce all healthcare-associated infections and not just one.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1756-994X
Relation: https://doaj.org/toc/1756-994X
DOI: 10.1186/s13073-024-01342-3
Access URL: https://doaj.org/article/389b70344adb4c468575cb18c6a2d66d
Accession Number: edsdoj.389b70344adb4c468575cb18c6a2d66d
Database: Directory of Open Access Journals
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  Value: <anid>AN0177062508;[969h]06may.24;2024May08.05:11;v2.2.500</anid> <title id="AN0177062508-1">Longitudinal analysis within one hospital in sub-Saharan Africa over 20 years reveals repeated replacements of dominant clones of Klebsiella pneumoniae and stresses the importance to include temporal patterns for vaccine design considerations </title> <p>Background: Infections caused by multidrug-resistant gram-negative bacteria present a severe threat to global public health. The WHO defines drug-resistant Klebsiella pneumoniae as a priority pathogen for which alternative treatments are needed given the limited treatment options and the rapid acquisition of novel resistance mechanisms by this species. Longitudinal descriptions of genomic epidemiology of Klebsiella pneumoniae can inform management strategies but data from sub-Saharan Africa are lacking. Methods: We present a longitudinal analysis of all invasive K. pneumoniae isolates from a single hospital in Blantyre, Malawi, southern Africa, from 1998 to 2020, combining clinical data with genome sequence analysis of the isolates. Results: We show that after a dramatic increase in the number of infections from 2016 K. pneumoniae becomes hyperendemic, driven by an increase in neonatal infections. Genomic data show repeated waves of clonal expansion of different, often ward-restricted, lineages, suggestive of hospital-associated transmission. We describe temporal trends in resistance and surface antigens, of relevance for vaccine development. Conclusions: Our data highlight a clear need for new interventions to prevent rather than treat K. pneumoniae infections in our setting. Whilst one option may be a vaccine, the majority of cases could be avoided by an increased focus on and investment in infection prevention and control measures, which would reduce all healthcare-associated infections and not just one.</p> <p>Keywords: AMR; Malawi; Genome surveillance; Healthcare-associated; Nosocomial infections; Surface antigens; Neonatal infection; Sepsis; Capsular polysaccharide; ESKAPE</p> <p>Supplementary Information The online version contains supplementary material available at https://doi.org/10.1186/s13073-024-01342-3.</p> <hd id="AN0177062508-2">Background</hd> <p>The burden of deaths in children under the age of five, including those due to neonatal sepsis, is especially high in low- and middle-income countries (LMICs) [[<reflink idref="bib1" id="ref1">1</reflink>]]; and one of the main pathogens causing these is <emph>Klebsiella pneumoniae</emph> (<emph>Kpn</emph>) [[<reflink idref="bib2" id="ref2">2</reflink>]]. Until the last decade, <emph>Kpn</emph> was typically readily treatable with beta-lactam antimicrobials, in particular third-generation cephalosporins (3GC). The effectiveness of 3GC is lost when these bacteria acquire extended-spectrum beta-lactamase enzymes (ESBLs). For <emph>Kpn</emph> there has been a drastic increase in 3GC-resistance by acquisition of ESBL enzyme-encoding plasmids; the proportion of <emph>Kpn</emph> hospital isolates that are ESBL-producing increased from 12% to > 90% in parts of sub-Saharan Africa (sSA) in the last decade [[<reflink idref="bib3" id="ref3">3</reflink>]]. The main remaining treatment option is carbapenems; these however are uncommonly available in sSA, rendering <emph>Kpn</emph> effectively untreatable in large parts of Africa. This is particularly problematic for neonatal sepsis caused by <emph>Kpn</emph>, and there is increasing evidence from large studies such as BARNARDS [[<reflink idref="bib2" id="ref4">2</reflink>]] and CHAMPS [[<reflink idref="bib4" id="ref5">4</reflink>]] that the burden of disease caused by <emph>Kpn</emph> falls most heavily on this patient group. Treatment options for neonates are even more limited than for other age groups as therapy needs to be intravenous and some antimicrobial classes are contraindicated, however, delay in establishing appropriate therapy is even more dangerous.</p> <p>The population structure of <emph>Kpn</emph>, similar to other opportunistic pathogens that thrive in the environment, is highly complex [[<reflink idref="bib5" id="ref6">5</reflink>]]. Understanding long-term population trends of clinically relevant lineages at both a local and global scale, and which antimicrobial resistance plasmids and elements they encode, is potentially relevant to guiding treatment, infection prevention strategies and vaccine development. However, few longitudinal studies of the <emph>Kpn</emph> population have been published so far, and the majority have come from collections established in high-income settings or South East Asia [[<reflink idref="bib6" id="ref7">6</reflink>]–[<reflink idref="bib10" id="ref8">10</reflink>]], whilst data from sub-Saharan Africa, South America and the Middle East and North Africa (MENA) remain sparse. Low-income countries in sub-Saharan Africa are particularly underrepresented in 'global' collections of pathogen genome data, despite often carrying the highest burden of disease [[<reflink idref="bib2" id="ref9">2</reflink>]].</p> <p>Whilst <emph>Kpn</emph> has a global distribution, there are clear geographical concentrations of dominant lineages and resistance genes, likely reflecting local antimicrobial pressure, patient populations in particular regarding comorbidities like HIV prevalence or malnutrition, and a range of other determinants. Developing a more representative picture of the global epidemiology of <emph>Kpn</emph> is relevant to the development of vaccines targeting <emph>Kpn</emph>, to ensure that the lineages and associated LPS O-antigens and capsular polysaccharide antigens most prevalent in countries with the highest burden of disease are targeted. A better understanding of the genomic epidemiology of <emph>Kpn</emph> in low-income countries is also crucial to advocate for access to antimicrobials, in particular newer agents, which is often profoundly limited by cost, leaving <emph>Kpn</emph> infections that can be readily treated in high- or middle-income countries de facto untreatable. Further, knowing at a facility level where problematic hotspots of transmission are within a hospital setting can help focus infection prevention and control (IPC) efforts.</p> <p>To address this urgent need for a better understanding of the <emph>Kpn</emph> population in sSA, we performed whole-genome sequencing on all bloodstream isolates phenotypically identified as <emph>Kpn</emph> from Queen Elizabeth Central Hospital (QECH) in Blantyre, Malawi, which has undertaken sentinel surveillance of bacteraemia and meningitis in partnership with the Malawi-Liverpool Wellcome Programme (MLW) since 1998 [[<reflink idref="bib3" id="ref10">3</reflink>]]. Our analyses include all isolates, irrespective of resistance profile or patient demographic, from 1996 to 2020, with no change in sampling strategy from 2000 to 2020. We report the shifts in resistance profile and population structure over time and highlight the relevance to consider trends over time and not just total numbers for considerations of new drug regiments or vaccine target selection.</p> <hd id="AN0177062508-3">Methods</hd> <p></p> <hd id="AN0177062508-4">Isolate collection</hd> <p>QECH is a government-run tertiary referral centre for the southern region of Malawi, which provides free care at the point of delivery. It serves a population of 800,000 for the area of urban Blantyre (Malawi census 2018). There are adult and paediatric A&Es, medical and surgical wards. There is a tertiary neonatal unit on-site and a separate paediatric surgical hospital which opened in 2017 (Fig. 2C; PICU, Paediatric HDU, Paediatric surgical ward).</p> <hd id="AN0177062508-5">Microbiological sampling and processing</hd> <p>Routine, (ISO15189 accredited since 2019) diagnostic blood culture services have been provided to the medical and paediatric wards by the Malawi-Liverpool-Wellcome Programme since 2000. For adults 7–10 mL of blood were collected from all patients admitted to the hospital with fever (axillary temperature > 37.5 °C) or clinical suspicion of sepsis, severe sepsis, or septic shock. Sepsis, severe sepsis, or septic shock were suspected in patients with tachycardia (≥ 90 beats per minute), hypotension (systolic blood pressure < 90 mm Hg), tachypnoea (respiratory rate > 20 per minute), or delirium. Three to 10 mL of blood was taken from children with non-focal febrile illness who tested negative for malaria, who were severely ill with suspected sepsis, or who failed initial malaria treatment and remained febrile. For adults and children with clinical suspicion of meningitis (temperature > 37.5 °C with seizures, headache, abnormal behaviour or meningism), a lumbar puncture was also performed, and the sample was sent for CSF analysis. For neonates with clinical signs of sepsis or meningitis (temperature > 37.5 °C, or other signs of clinical deterioration such as reduced activity, increasing work of breathing or respiratory rate > 60, heart rate > 180 or < 100, and unexplained seizures) a blood culture and CSF sample was collected. Afebrile patients were unlikely to have had blood or CSF cultures unless the clinical suspicion for sepsis or meningitis was high.</p> <p>For blood cultures, samples were collected using aseptic methods and inoculated into a single aerobic bottle (BacT/Alert, bioMérieux, Marcy-L'Etoile, France). These were incubated using the automated BacT/Alert system (bioMérieux, France) since 2000. Before which, they were cultured manually. Samples that flagged positive were Gram-stained and Gram-negative bacilli are identified by the Analytical Profile Index (Biomérieux). CSF samples were processed for cell count and biochemistry before manual culture. Antimicrobial susceptibility testing was determined by the disc diffusion method (Oxoid, UK) using AST breakpoints that were current at the time, initially following the relevant version of the British Society of Antimicrobial Chemotherapy's guidelines until 2018, after which time EUCAST methods and breakpoints were introduced. Organisms which showed reduced inhibition when exposed to ceftriaxone 30 mg discs, ceftriaxone 5 mg discs, or cefpodoxime 10 mg discs were classified as resistant to ceftriaxone. Intermediate isolates were classified as resistant to ceftriaxone. Details for the identification of other organisms are described elsewhere [[<reflink idref="bib3" id="ref11">3</reflink>]]. <emph>Klebsiella pneumoniae</emph> isolates were stored at − 80 °C on microbank beads.</p> <hd id="AN0177062508-6">Whole genome sequencing and QC</hd> <p>All <emph>Klebsiella pneumoniae</emph> isolates that could be recovered from the start of the MLW archive to April 2020 were thawed and incubated on MacConkey's media for 18–24 h at 37 °C. Plates with pure growth then had a single colony pick taken and inoculated into 15 ml of buffered peptone water for 18–24 h at 37 °C. These samples were then centrifuged and the supernatant was discarded. The pellet was then resuspended in buffer and the DNA was extracted using the QIAsymphony machine and QIAsymphony DSP kit with onboard lysis, according to the manufacturer's instructions. Quality control was done using Qubit and samples with a DNA volume of less than 200 ng were repeated. DNA was sequenced at the Wellcome Sanger Institute on the Illumina HiSeq X10 instrument (Illumina Inc., USA) to produce 150-bp paired-end reads.</p> <p>Eleven out of 1313 samples failed sequencing; six of these were below the Sanger-internal threshold for minimum DNA amount (0.2 µg total); these eleven samples were removed from further analyses. Quality control on all remaining isolates was performed using species confirmation via Kraken v0.10.6 [[<reflink idref="bib11" id="ref12">11</reflink>]] to identify contaminants (incorrect species). Any species assignment other than <emph>Klebsiella pneumoniae</emph>, <emph>Klebsiella variicola</emph>, unclassified and synthetic construct were considered contaminants; the latter two categories frequently derive from plasmid sequences in our experience. All isolates with higher than 10% of other species were removed as contaminated (Fig. S2A). Similarly, isolates with a ratio of heterozygous SNPs over 5% were removed as likely contaminated by a related strain (Fig. S2B). We note that four sequence runs; 42092_2#162, 42070_2#207, 42092_2#170, 42092_2#237; include a higher proportion of 'unclassified' reads, whose origin is unlikely from a bacterial contaminant but might be from phages, unknown plasmid sequences, or other unclassified components, leading to larger then usual assembly size (> 6 Mb; Table S1). Further quality control used the assemblies, where all isolates resulting in more than 500 contigs were removed as likely contaminated by other species or strains, or of very low quality (Fig. S2C). De novo assembly of the remaining 1156 genome sequences was performed using SPAdes v3.14.0 [[<reflink idref="bib12" id="ref13">12</reflink>]] incorporated into the bacterial genome assembly pipeline described previously [[<reflink idref="bib13" id="ref14">13</reflink>]]. Annotation of all assemblies was performed using prokka [[<reflink idref="bib14" id="ref15">14</reflink>]] v1.14.5.</p> <hd id="AN0177062508-7">Long-read sequencing and hybrid assemblies</hd> <p>To quality-control our short-read DNA extraction, three selected strains, representing major sequence types and complex plasmid combinations, were used for long-read sequencing. DNA was isolated using the MasterPure Complete DNA and RNA isolation kit to retrieve long fragments, and sequencing was performed using the Oxford Nanopore MinION platform with R9 flow cells. Base calling was performed using guppy (v6.5.7, https://community.nanoporetech.com), and the initial assembly was performed with flye (v2.9.2, [[<reflink idref="bib15" id="ref16">15</reflink>]]). The assemblies were then polished with medaka (v1.8.0, https://github.com/nanoporetech/medaka) which uses the long read sequence data followed by polypolish (v0.5.0, [[<reflink idref="bib16" id="ref17">16</reflink>]]) including the insert size filter step as recommended by the author. The assemblies were then annotated using prokka as above, and plasmid replicons in these assemblies were determined by submitting to plasmidfinder [[<reflink idref="bib17" id="ref18">17</reflink>]] via the online platform (https://cge.food.dtu.dk/services/PlasmidFinder/; software version 2.0.1; database version 2023–01-18). Accession numbers and contig details are provided in Table S6.</p> <hd id="AN0177062508-8">Genome analyses and phylogenetics</hd> <p>For comparative analyses, isolates from recent studies at QECH were included, as they are part of the bloodstream collection [[<reflink idref="bib18" id="ref19">18</reflink>]–[<reflink idref="bib20" id="ref20">20</reflink>]]. Details of all sequences used in the analyses are given in Supplementary Table 1. Resistance gene prediction and plasmid replicon types were performed using ariba [[<reflink idref="bib21" id="ref21">21</reflink>]] (v2.14.6) and the srst2-argannot [[<reflink idref="bib22" id="ref22">22</reflink>]], CARD [[<reflink idref="bib24" id="ref23">24</reflink>]] and plasmidfinder [[<reflink idref="bib17" id="ref24">17</reflink>]] databases (download date 14.02.2022). To assess aminoglycoside resistance; ANT3 is grouped by ariba as AacAad_AGly, AadA4_5_AGly, AadA_AGly, AadA_AGly_2, AphA6_AGly; AAC(<reflink idref="bib3" id="ref25">3</reflink>)-II is grouped by ariba as Aac3_IIa_AGly; ANT(2″)-I is grouped by ariba as AadB_AGly and AAC(6′)-I is grouped by ariba as AacAad_AGly_3.</p> <p>Prediction of sequence types, (sub)species and <emph>Klebsiella</emph>-specific virulence factors were performed using kleborate v2.1.0 [[<reflink idref="bib25" id="ref26">25</reflink>]] and the –r setting; the results are given for all isolates in Table S1. O- and K-types were predicted with kaptive [[<reflink idref="bib26" id="ref27">26</reflink>]] (v2.0.6) which incorporates O-loci as well as O-serotype prediction [[<reflink idref="bib26" id="ref28">26</reflink>]–[<reflink idref="bib28" id="ref29">28</reflink>]]; and we added an additional option of <emph>wbbZ</emph> as extra gene to distinguish between O1a (O1/O2 + <emph>wbbY</emph>, no <emph>wbbZ</emph>) and O1b (O1/O2 + <emph>wbbY</emph> + <emph>wbbZ</emph>) as described recently [[<reflink idref="bib29" id="ref30">29</reflink>]], adding <emph>wbbZ</emph> as implemented in kaptive versions before v2 [[<reflink idref="bib27" id="ref31">27</reflink>]] and expanding the.logic file to distinguish O1a/b. As the <emph>wbbZ</emph> gene is located outside the O-locus, we additionally searched for this gene (reference sequence LT174607.1:7925–8734) using ariba as described above.</p> <p>Core genome SNPs for <emph>K. pneumoniae</emph> subsp. <emph>pneumoniae</emph> isolates were determined using snippy [[<reflink idref="bib30" id="ref32">30</reflink>]] (v4.6.0; https://github.com/tseemann/snippy) with the <emph>Klebsiella pneumoniae</emph> subsp. <emph>pneumoniae</emph> NTUH-K2044 genome (AP006725.1) [[<reflink idref="bib31" id="ref33">31</reflink>]] as reference. Recombinant regions were removed using gubbins [[<reflink idref="bib32" id="ref34">32</reflink>]] (v3.2.1) and the FastTree [[<reflink idref="bib33" id="ref35">33</reflink>]] option ('-tree-builder fasttree'), given the very high number of samples, as implemented in gubbins; and only isolates from sequence types with more than one isolate in our dataset were included in the analysis to further reduce compute time. The same strategy was used for <emph>K. quasipneumoniae</emph> and <emph>K. variicola</emph> using strains ATCC 700603 (CP029597.1) [[<reflink idref="bib34" id="ref36">34</reflink>]] and At-22 (CP001891.1) [[<reflink idref="bib35" id="ref37">35</reflink>]] as reference, respectively, where all isolates were included and default options for gubbins were used (RaxML for tree generation, given the much smaller sample numbers). A snp-only alignment was generated from the recombination-free gubbins output using snp-sites [[<reflink idref="bib36" id="ref38">36</reflink>]] v2.5.1 with the -c option (selecting for ACGT-only) for <emph>K. quasipneumoniae</emph> and <emph>K. variicola</emph>; for <emph>K. pneumoniae</emph> subsp. <emph>pneumoniae</emph>, given the high variation of isolates, the -c option was too stringent (resulting in a < 200-bp alignment) and we thus used snp-sites to isolate all snps but without selecting for ACGT-only sites.</p> <p>The snp-only alignments were used as input for iqtree [[<reflink idref="bib37" id="ref39">37</reflink>]] (v1.6.12), the number of constant sites was determined with snp-sites -C function from the original full alignment and provided to the '-fconst x,x,x,x' iqtree function. The model was determined with the integrated ModelFinder [[<reflink idref="bib38" id="ref40">38</reflink>]] function '-m TEST' resulting in GTR + F + I + G4, GTR + F + I + G4, TVM + F + I + G4, for the alignments from <emph>K. pneumoniae</emph>, <emph>K. quasipneumoniae</emph> and <emph>K. variicola</emph>, resp. We set '-keep-ident' to keep identical data and '-bb 1000' for 1000 ultrafast bootstrap replicates. The resulting trees were then adjusted to SNP-length branch lengths using the python implementation of pyjar [[<reflink idref="bib39" id="ref41">39</reflink>]] (https://github.com/simonrharris/pyjar) as input for rPinecone [[<reflink idref="bib40" id="ref42">40</reflink>]]. Closely related clusters taking their phylogenetic distance into account were determined with 5 SNPs as cut-off for minor and 10 as cut-off for major clusters. These were visualised with iTol [[<reflink idref="bib41" id="ref43">41</reflink>]] (v6.8) with visualisation files generated as implemented in rPinecone [[<reflink idref="bib40" id="ref44">40</reflink>]]. For ST35, ST39 and ST14/15 (combining ST14 and ST15 isolates in one analysis) we performed ST-specific mappings of all respective isolates against references from these sequence types to gain higher resolution (CP041353.2 [[<reflink idref="bib42" id="ref45">42</reflink>]], LR991401.1 [[<reflink idref="bib43" id="ref46">43</reflink>]] and CP008929.1 [[<reflink idref="bib44" id="ref47">44</reflink>]], resp.).</p> <p>Pan-genome reconstruction, generation of a core gene alignment, and identification of neighbouring gene regions were derived via panaroo [[<reflink idref="bib45" id="ref48">45</reflink>]] (v1.3.3) using the '–clean-mode strict' setting. The phylogenetic tree based on the core gene alignment, with informative sites extracted with snp-sites as above, was calculated using iqtree [[<reflink idref="bib37" id="ref49">37</reflink>]] (v1.6.12) and the internal ModelFinder [[<reflink idref="bib38" id="ref50">38</reflink>]] function (GTR + F + ASC + G4) as above. All other metadata summary analyses and visualisation were generated using the ggplot [[<reflink idref="bib46" id="ref51">46</reflink>]] and ggtree [[<reflink idref="bib47" id="ref52">47</reflink>]] packages in R [[<reflink idref="bib48" id="ref53">48</reflink>]] unless indicated otherwise; some colour schemes are derived from or inspired by the MetBrewer (https://github.com/BlakeRMills/MetBrewer) and NationalParks (https://github.com/katiejolly/nationalparkcolors) R libraries. Composite figures were generated using the patchwork package (https://github.com/thomasp85/patchwork). An RMarkdown file to generate the analysis plots and the underlying tree and data files are freely available online (https://github.com/EvitaH/QECHospitalKlebs; https://doi.org/10.5281/zenodo.8421658).</p> <hd id="AN0177062508-9">Results</hd> <p></p> <hd id="AN0177062508-10">The QECH bloodstream isolate collection</hd> <p>QECH is the main referral hospital for the southern region of Malawi and the main hospital for Blantyre, the second largest city of Malawi (population 800,024 at the 2018 census). It has approximately 1000 beds and receives around 10,000 adult medical and 30,000 paediatric medical admissions per year. A quality-assured diagnostic microbiology service was initially implemented in partnership with MLW at QECH in 1998. Laboratory blood culture was initially manual, and an automated system was implemented in 2000 (BacT/ALERT, Biomerieux). The diagnostic microbiology service expanded to culture cerebrospinal fluid (CSF) from 2002. During the study period, all adult and paediatric medical patients with suspected sepsis or meningitis were eligible for blood and/or CSF culture at the discretion of the admitting physician, and where possible before the administration of antimicrobials. Bacterial isolates were identified by standard techniques detailed in the methods.</p> <p>At the start of the period, the first-line treatment against bloodstream infections was crystal-penicillin and chloramphenicol although gentamicin was used in neonates instead of chloramphenicol. This began to change with the introduction of the 3rd-generation cephalosporin ceftriaxone from 2004, which was rapidly adopted as a first-line agent by the Department of [adult] Medicine and more slowly by the Department of Paediatrics, which continues to use penicillin and gentamicin in the management of neonatal sepsis, as per WHO guidelines [[<reflink idref="bib49" id="ref54">49</reflink>]].</p> <p>Members of the order Enterobacterales including <emph>Kpn</emph> were identified by API biochemical index (Biomerieux, France). <emph>Klebsiella pneumoniae</emph> is known to comprise a species complex including <emph>Klebsiella pneumoniae subsp. pneumoniae</emph> as well as several closely related species like <emph>K. variicola</emph> and <emph>K. quasipneumoniae</emph> and their respective subspecies [[<reflink idref="bib50" id="ref55">50</reflink>]]. These can only be identified to species complex level via API, and we therefore refer to all members of the <emph>K. pneumoniae</emph> species complex as <emph>Kpn</emph> and specify when referring to specific species (Fig. 1A).</p> <p>Graph: Fig. 1 Kpn cases at QECH. A Numbers of Kpn cases per year at QECH. Bars represent the crude frequency of Kpn infection for each year from 1998 to 2021, with the different colours representing the different age groups of the patients. B Blood culture positivity rate (per 1000 blood cultures) of Kpn in neonates and the entire patient population. C A diagrammatic overview of the samples included in this study (created with <ulink href="http://www.biorender.com">www.biorender.com</ulink>)</p> <hd id="AN0177062508-11">Dramatic increase in Kpn cases from 2016</hd> <p>The crude frequency of <emph>Kpn</emph> cases was high at the start of the period with 121 cases in 1998, although this overlapped with a year of active surveillance of febrile adult patients [[<reflink idref="bib51" id="ref56">51</reflink>]] and we thus focused on longitudinal analyses from 2000 onwards. The number of cases from 2000 to 2015 was stable with a mean of 44 cases per year (Fig. 1A). This increased to a mean of 187 cases per year from 2016 to 2021 with a peak of 279 in 2019.</p> <p>Neonatal infection with <emph>Kpn</emph> accounted for 39.3% of all <emph>Kpn</emph> infections, even though only 11.2% of all blood cultures were taken from this patient group. The overall rate of <emph>Kpn</emph> isolation was 24.9 per 1000 blood cultures for neonates, 4.3 per 1000 blood cultures for other patients (rate ratio of neonates vs non-neonates 5.11 (95% CI 4.66–5.61)). The increase in <emph>Kpn</emph> cases post 2016 was driven by an increase in neonatal infections (Fig. S1A–1G); there were peaks in the <emph>Kpn</emph> blood culture isolation rate in neonates in 2008, 2014 and 2017 which suggest outbreaks or ongoing transmission on the neonatal unit (Fig. 1B), and our genomic analyses further support hospital transmission given the close genetic relationships of the respective isolates. Though the blood culture positivity rate was increased for most age groups in the period from 2016 to 2021, for neonates this increase was particularly marked (33.5 per 1000 blood cultures in the period 2016–2021 compared to 15.4 per 1000 blood cultures in the period 1998–2015; Fig. S1A–1D). As well as the peak in neonatal cases, there was also a much smaller peak in the adult age group, which shifted from the 30–35 year age group in the period from 1998 to 2015 to the 35–40 year age group in the period from 2016 to 2021 (Fig. S1E–1H).</p> <hd id="AN0177062508-12">Increase in Kpn numbers driven by temporally defined expansions of sequence types (STs)</hd> <p>We retrieved 1313 isolates not previously sequenced from the QECH <emph>Kpn</emph> collection, 1302 of which could be recovered for growth and yielded enough DNA for sequencing; and 1156 of these passed all quality control steps for further analysis (for details, see the ' Methods' section; Fig. S2). In addition, we included 217 samples from previously published studies of this collection [[<reflink idref="bib18" id="ref57">18</reflink>]–[<reflink idref="bib20" id="ref58">20</reflink>]], resulting in 1373 sequenced genomes analysed in this study (Fig. 1C).</p> <p>The majority (1274/1373, 92.8%) belong to <emph>Klebsiella pneumoniae</emph> subsp. <emph>pneumoniae</emph> with only very small proportions representing other (sub)species, including <emph>K. quasipnemoniae</emph> subsp. <emph>quasipneumoniae</emph> (10/1373, 0.7%), <emph>K. quasipneumoniae</emph> subsp. <emph>simillipneumoniae</emph> (46/1373, 3.4%) and <emph>K. variicola</emph> subsp. <emph>variicola</emph> (43/1373, 3.1%). Based on phenotypic microbiology data (Fig. 1A and B) we observed a drastic increase in isolates from neonatal infection in 2016/2017 which is reflected in our sequenced collection.</p> <p>Assessing the diversity of STs over time highlights that the increase in <emph>Kpn</emph> infections represents repeated increase and subsequent decrease in numbers of different multilocus sequence types (Fig. 2A). Whilst the earlier years show a diverse composition of STs with small numbers of each (<emph>n</emph> < 15 total), the increase in total <emph>Kpn</emph> numbers is mainly driven by one or two major STs, with these peaks spanning approximately 1.5 to 2 years each. We furthermore observe that after the increase of isolates of the major STs, each of them reverts to their original very low numbers (Fig. 2A).</p> <p>Graph: Fig. 2 Uneven distribution of STs and cases at QECH wards indicate hospital-acquired infections. A Main STs and B wards the samples were derived from over time. C A schematic map of QECH with the wards samples of our study were derived from highlighted. AETC, Adult emergency and Trauma Centre; Paediatric A&E, paediatric accident and emergency; PICU, paediatric intensive care unit; HDU, high dependency unit</p> <p>A previous study [[<reflink idref="bib19" id="ref59">19</reflink>]] highlighted an outbreak of ST340 across the neonatal unit which is reflected here, where it comprises 31 out of 105 isolates in 2013 and 2014 (29.5%; Fig. 2A). We then see an increase of the numbers of ST14 (19/44 in 2015; 43.2%) which is present at a lower proportion before and after this period; followed by an increase in ST25 (13/87 in 2016; 14.9%) which again is present at lower proportions before and after. The year 2017 then saw a steep increase in <emph>Kpn</emph> infections overall; mainly driven by an expansion of ST39 which comprises more than half the samples at the time (100/188 in 2017; 53.2%). This clone however again retreats back to low numbers, with a few lineages dominating, in particular, ST1552 (35/208 in 2018; 16.8%) and ST35 (40/208 in 2018; 19.2%). The year 2019 then sees another increase in ST14 (61/236 in 2019; 25.8%) as well as ST15 (30/236 in 2019; 12.7%), which is a known high-risk clone predominantly found in South East Asian collections where it has been linked to an early spread of carbapenem resistances via <emph>bla</emph><subs>NDM</subs> genes [[<reflink idref="bib52" id="ref60">52</reflink>]]. Interestingly, ST307, a major resistance clone highlighted in other parts of the world [[<reflink idref="bib54" id="ref61">54</reflink>]], only formed a small proportion with only a single year of over 10% (3/28 in 2013; 10.2%).</p> <hd id="AN0177062508-13">Isolation of Kpn from neonatal and paediatric wards account for observed changes, whilst adul...</hd> <p>The patterns over time differ significantly between wards (Fig. 2B), suggesting the increase in <emph>Kpn</emph> infections could represent a pattern of repeated ward-specific outbreaks, rather than a generalised increase across all wards (Fig. 2B). This pattern of ward-focused increases in isolate numbers was repeatedly observed on the Chatinkha neonatal unit and the surgical PICU/HDU, where most of the changes in numbers of isolates occur. An indication of local outbreaks is further supported by analysing the major STs, which increase at defined times in specific wards (Fig. 2B and C; Fig. S3). To investigate the impact of closely related isolates (< 5 core SNP distance) that are likely within-hospital transmissions on the number of infections overall, we analysed a core genome tree for each of the three species and identified closest-neighbour SNP distances in a root-to-tip approach [[<reflink idref="bib40" id="ref62">40</reflink>]] (Fig. 3). This highlighted that most highly clonal lineages (defined as < 5 SNPs) are derived from <emph>K. pneumoniae</emph> subsp. <emph>pneumoniae</emph> (Fig. 3A) representing 402 of 1165 isolates as a conservative estimate based on whole-species analyses, with 43% of the <emph>K. quasipneumoniae</emph> subsp. <emph>similipneumoniae</emph> (20 of the 46 isolates in 6 lineages, Fig. 3B) and 25% of the <emph>K. variicola</emph> isolates (10 of the 40 isolates of this species, Fig. 3C); and no < 5 SNP-related isolates of <emph>K. quasipneumoniae</emph> subsp. <emph>quasipneumoniae</emph>.</p> <p>Graph: Fig. 3 Most cases suspected to be caused by within-ward transmissions. Mapping trees against reference genomes for A K. pneumoniae subsp. pneumoniae excluding STs represented with only a single isolate in the dataset, B K. quasipneumoniae and C K. variicola. D and E show ST-specific mapping of the relevant isolates against references for ST35 (D), ST39 (E), and ST14/ST15 (F). The coloured tips indicate isolates with < 5 SNPs distance to their nearest neighbour based on a root-to-tip approach to identify related clusters of isolates [[<reflink idref="bib40" id="ref63">40</reflink>]]. The colour ring in A shows the main STs, the columns in D–F show the year of isolate (left column) and major ward (right column)</p> <p>Despite removing the single-isolate STs for this analysis, our dataset still contains a high number of <emph>K. pneumoniae</emph> subsp. <emph>pneumoniae</emph> STs, which results in a highly diverse dataset. As this diversity has potential to impact on tree resolution depending on the reference used [[<reflink idref="bib55" id="ref64">55</reflink>]], we investigated three key STs in more detail. We focused on ST35, ST39, and the closely related ST14 and 15 together, whose pattern of accumulation in a ward and year strongly indicates multiple hospital-associated transmissions (Fig. S3), in more detail by mapping against strain-specific reference genomes (Fig. 3A). ST39 and ST35 are two key STs when considering the per-year breakdown of isolates per ward for the neonatal unit (Chatinka; Fig. S3); whilst ST14 and ST15 were identified in high numbers in the PICU/HDU in the paediatric surgical ward (Fig. S3). Here, we can observe that 77% of the isolates (51/55 ST35, 110/136 ST39, 182/254 ST14-15) have their nearest neighbour within the threshold of 5 SNPs; representing 100% (17/17) of ST35 and 88.9% (80/90) of ST39 from Chatinka and 88% (57/65) ST14 and 15 from the PICU/HDU (Fig. 3A). This high relatedness of isolates strongly supports within ward transmission and the argument that <emph>Kpn</emph> is a healthcare-associated cause of neonatal sepsis. Whilst details like length of stay and within-ward location are not available, the fact that patients are regularly transferred from Chatinkha to the PICU/HDU if surgical procedure is required means that these wards are already linked by a clear transmission path via patients, and several potential between-ward transmission events are indicated in particular in ST35 and ST14/15 (Fig. 3D, F).</p> <p>Over the period from 2015 to 2018, there was an increased focus on performing blood cultures to ensure every neonate on Chatinkha nursery that met the clinical criteria for suspected neonatal sepsis was included, which may explain some of the large increase in <emph>Kpn</emph> cases in 2017 on the nursery. Establishing optimal infection prevention and control (IPC) has been an ongoing challenge [[<reflink idref="bib56" id="ref65">56</reflink>]]; specifically, handwashing has been difficult due to intermittent water supply and a lack of access to soap or hand sanitiser and there are limited numbers of cots, so neonates have often shared them. In response to the large increase in <emph>Kpn</emph> cases, there was a renewed focus on IPC, hand sanitiser and soap procurement was prioritised, deep cleans of the ward environment were performed regularly, there was increased cleaning of ward surfaces, ward traffic was limited, wooden cots are being replaced with easier to clean plastic ones, the number of neonates sharing a cot was reduced and oxygen delivery devices are being replaced with single-use items.</p> <hd id="AN0177062508-14">Antimicrobial resistance and implications for treatment</hd> <p>Phenotypic resistance profiles of the strains, where available, mirror the very concerning trends reported previously for <emph>Kpn</emph> in QECH [[<reflink idref="bib3" id="ref66">3</reflink>]] (Fig. S4, Table S4). Resistance against the frontline treatments is widespread; a particular concern is a high proportion of isolates with phenotypic resistance to ceftriaxone (83.6% in 2019), gentamicin (79.3% in 2019) and cotrimoxazole (93.0% in 2019), which rapidly emerged from 2012 onwards with very little change over time (Fig. S4A–C; > 75% of tested isolates were resistant to these agents each year 2012–2020). We notice some variation in co-amoxiclav (augmentin: amoxicillin + clavulanic acid) susceptibility profiles but from 2019 again show > 75% of tested isolates as resistant (Fig. S4A). Importantly, we notice that a trend of increased numbers of chloramphenicol susceptible isolates (Fig. 4B, Fig. S4G), which has previously reported in Malawi [[<reflink idref="bib3" id="ref67">3</reflink>]], is also explicit in our <emph>Kpn</emph> collection in particular in recent isolates (38.7%, 51.5%, 72.2% isolates sensitive for 2018–2020). The presence of <emph>catA1</emph> or <emph>catA2</emph> agrees well with phenotypic resistance whereas the presence of <emph>catB4</emph> did not as shown in Fig. 4B; where we see a large number of isolates with sensitive phenotype, no <emph>catA1/2</emph> but <emph>catB4</emph>, whereas only a small fraction of isolates has resistant phenotype, no <emph>catA1/2</emph> but <emph>catB4</emph> where we hypothesise a different mechanism is likely driving the resistant phenotype. For isolates with resistant phenotype and encoding both <emph>catA1/2</emph> and <emph>catB4</emph>, the resistance is presumed to be driven by <emph>catA1/2</emph>. This agrees with <emph>catB4</emph> representing a non-functional truncated variant of <emph>catB3</emph> as was recently reported [[<reflink idref="bib57" id="ref68">57</reflink>]]. We furthermore note a sensitivity of 50% or higher in the majority of years measured in ciprofloxacin (6/9 years from 2012 to 2020, including 2017, 2018 and 2020; Fig. S4E, Table S4), which together with chloramphenicol thus represent two antimicrobials that might thus be valid treatment options.</p> <p>Graph: Fig. 4 Resistance mechanisms only explain beta-lactam, not aminoglycoside, phenotypes. A The distribution of ESBL genes over time illustrating the dominance of blaCTX-M-15. B Phenotypic chloramphenicol resistance profiles proportional over time and the presence or absence of catA1 and/or catA2 compared to catB4, the recently re-characterised non-functional CAT enzyme. C and D Predicted genotypic aminoglycoside resistance was based on the presence of an Aac6′-Ib-cr or Aac6′-Ib gene (AAC(6′)-I) for amikacin and Aac3_IIa (AAC [[<reflink idref="bib3" id="ref69">3</reflink>]]-II) or AadB (ANT(2″)-I) for gentamicin. The x-axis shows the samples based on phenotypic resistance profiles (intermediate, sensitive, resistant) against C amikacin and D gentamicin, and the bars show the number of isolates in the respective category that would have been predicted as sensitive (blue) or resistant (red) based on the genes as above</p> <p>Resistance to 3GC was widespread (83% resistance to cefpodoxime/ceftriaxone by 2020; Fig. S4A) and almost exclusively driven by the acquisition of the <emph>bla</emph><subs><emph>CTX-M-15</emph></subs> gene, which continuously outcompetes other ESBL-encoding genes that are occasionally present in the <emph>Kpn</emph> population, but which are only sporadically detected (Fig. 4A, Table S2). The number of plasmid replicons per isolate stabilised after the first increase in numbers around 2015, highlighting that a few large AMR plasmids are likely responsible for the majority of AMR-isolates (Fig. S5C, Fig. S6B, FigS7B, Fig. S8A, Table S3). Strains with a moderate number of plasmid replicons dominate the population (0–5) and this remains stable over time. Only a small number of isolates with more than five plasmid replicons associated was identified at any point in time over the whole time period investigated (Fig. S8); and using long-read sequencing on two selected isolates from major STs confirms the predicted low number of plasmid replicons and thus also plasmids (Fig. S9), again highlighting that the repertoire of resistance plasmids that led to the success of <emph>Kpn</emph> in this setting was driven by the change to a predominantly ESBL<sups>+</sups> population as the key event, with the population then remaining stable over time with respect to plasmid diversity and numbers of resistance genes (Fig. S8B).</p> <p>Carbapenem resistance genes are still only rarely observed; we identified two samples encoding for <emph>bla</emph><subs><emph>NDM-1</emph></subs>; one in a ST39 in 2016 and one in a ST45 in 2019, perhaps unsurprisingly as carbapenem usage is still comparatively unusual. We also investigated resistances against other potential alternative treatment options colistin, fosfomycin and tigecycline. Resistance to these agents is all at low levels, with only one fosfomycin resistance gene detected (<emph>fosA</emph>) and none against colistin or tigecycline predicted in our dataset, and one isolate with predicted mutations that can confer resistance to colisin (<emph>pmrB</emph>), indicating that these might be viable alternatives for ESBL<sups>+</sups> infections at QECH, although tigecycline is contraindicated in children under 8y (Table S2) and neither agent ideal treatments for bacteraemia [[<reflink idref="bib58" id="ref70">58</reflink>]]. Tigecycline resistance is often conferred by upregulation of transport proteins and thus challenging to predict and has also been observed during within-patient evolution [[<reflink idref="bib60" id="ref71">60</reflink>]–[<reflink idref="bib62" id="ref72">62</reflink>]]; we could however not detect the described plasmid-derived antimicrobial resistance gene <emph>tetX</emph> in our collection [[<reflink idref="bib63" id="ref73">63</reflink>]].</p> <p>Based on the AMR phenotype identified by disc diffusion, amikacin remains a viable alternative to gentamicin, although there are concerns over blood–brain barrier penetration in cases of meningitis and renal toxicity in settings where therapeutic drug monitoring is not possible. Phenotypic data are corroborated by a more detailed analysis of the aminoglycoside AMR genes (Fig. 4C and D). In total 90.5% of all isolates (1243/1373) are predicted to encode one or more aminoglycoside resistance genes, however, the majority are predicted to confer resistance to streptomycin (ANT3) and gentamicin (AAC(<reflink idref="bib3" id="ref74">3</reflink>)-II; ANT(2″)-I). Figure 4D highlights these as 'predicted resistant' (ANT3 for streptomycin, AAC(<reflink idref="bib3" id="ref75">3</reflink>)-II and/or ANT(2″)-I for gentamicin). Only a fraction of isolates (43.2%, 593/1373) encoded AAC(6′)-I; which can provide resistance against amikacin; the presence of this gene is interpreted as 'predicted resistant' in Fig. 4C. Aminoglycoside resistance is known to be challenging to predict [[<reflink idref="bib64" id="ref76">64</reflink>]]; whilst phenotypic gentamicin resistance testing matches very well with the predicted profile based on acquired genes, the presence of AAC(6′)-I does not by itself confer resistance in a large number of isolates, with only 12 isolates encoding for either of these, representing 2%, actually showed a resistant phenotype (Fig. 4D; Table S4).</p> <hd id="AN0177062508-15">Potential vaccine target stability</hd> <p>The <emph>Kpn</emph> polysaccharide antigens are of high interest as vaccine targets; in particular the large capsular antigen. As expected, we observed a very high number of different K-loci (90 predicted), as well as a diverse set of O-loci (15 predicted), the latter are known to be far less diverse in <emph>Kpn</emph> [[<reflink idref="bib28" id="ref77">28</reflink>]]. Recent findings furthermore showed that <emph>wbbZ,</emph> encoded on the chromosome outside the O-locus, contributes to the diversity of the O-Ag structure displayed by <emph>Kpn</emph> and thus two isolates with the same O-locus can still result in different O-types based on the presence or absence of this gene<emph>.</emph> Immunologically relevant differences in O-Ag thus require the prediction of the O-Ag locus and <emph>wbbZ</emph> [[<reflink idref="bib29" id="ref78">29</reflink>]], so we added this to our O-Ag type predictions (Fig. S10A–D) where we thus distinguish between O1a and O1b as described by Kelly et al<emph>.</emph> [[<reflink idref="bib29" id="ref79">29</reflink>]]. Reflecting the trend observed with the ST types, we observe temporal variation in the prevalence of O-types and K-types (Fig. 5A and D, Fig. S10A). The non-ESBL population again displays a much greater diversity for both types of antigen (Fig. 5B and E, Fig. S10B), whilst the ESBL population is more biased towards dominant O- and K-types (Fig. 5C and F, Fig. S10C).</p> <p>Graph: Fig. 5 High fluctuation of putative vaccine targets over time and diversity within major STs. The distribution of O-Ag locus types (top panels) and K-locus types (lower panels), resp., over time showing total numbers (A and D); and proportions of only isolates with ESBL resistance genes (B and E) or ESBL-negative isolates (C and F)</p> <p>Beyond the dominant O1b (822/1373; 59.9%) and O2afg (184/1373; 13.4%) predicted O-antigen types, the infrequently observed type O4 comprises a substantial fraction of isolates (90/1373; 6.6%); and 35 isolates have a prediction accuracy as 'Low'or 'None' which can indicate either low assembly quality or novel O-Ag loci and types. In addition, we note two isolates with unknown O-Ag locus encoding for additional <emph>wbbY</emph> and <emph>wbbZ</emph> genes, indicating that more variation might be within possibly novel O-Ag loci depending on additional enzymes encoded in the chromosomal backbone (Table S5). We furthermore note 8 isolates with O1/O2 loci encoding for only <emph>wbbZ</emph> (not <emph>wbbY</emph>); whilst the recent work showed no structural differences in the O-Ag if <emph>wbbZ</emph> alone was added, we cannot exclude other carbohydrate enzymes on the chromosomes of our isolates that might lead to additional different structures if combined with <emph>wbbZ</emph> (Table S5). Of relevance, we also observe variation of O- and K-type combinations within the same sequence type (Fig. 5G and H, Fig. S10D) and with each other (Fig. 5I, Fig. S10E).</p> <p>Considering hypothetical vaccine strategies and constructs, an efficacious maternally administered quadrivalent O-antigen-based vaccine (O1a, O1b, O2afg, O4; including O1a as O1b-enconding isolates can switch between O1a and O1b [[<reflink idref="bib29" id="ref80">29</reflink>]]) would be expected to protect against up to 89% of cases of neonatal sepsis in Blantyre (345/388), whereas a K-antigen based vaccine would require 22 K-antigens to be represented to protect against an equal number of cases of neonatal sepsis when taking the entire timespan of our study into account (KL10, KL102, KL103, KL104, KL106, KL108, KL117, KL142, KL15, KL16, KL17, KL19, KL2, KL23, KL25, KL30, KL43, KL48, KL51, KL52, KL62, KL9); a quadrivalent K-antigen based vaccine (KL2, KL25, KL30, KL102) would cover 58% (198/388) of cases. We furthermore observe no regular cycling between a specific set of K-types, but rather that different K-types regularly emerge associated with major clones (Fig. 5). Given current knowledge of > 180 predicted K-types [[<reflink idref="bib26" id="ref81">26</reflink>]] and > 70 determined structures so far [[<reflink idref="bib65" id="ref82">65</reflink>]–[<reflink idref="bib67" id="ref83">67</reflink>]] (likely an underestimation), and the propensity of the K-locus to recombine, it will be very challenging to predict how long a K-type vaccine would remain effective.</p> <hd id="AN0177062508-16">Trends of resistance vs virulence determinants</hd> <p>Whilst most <emph>Kpn</emph> isolates are opportunistic pathogens with poorly understood virulence profiles, the hypermucoid phenotype is a known virulence determinant arising from hyperproduction of capsule and causing a different clinical infection type, i.e. liver abscess and meningitis in otherwise healthy individuals, often progressing very rapidly and with poor prognosis. The capsule hyperproduction is usually driven by acquisition of a virulence plasmid encoding for the capsule polysaccharide upregulation factor <emph>rmpA2</emph> as well as several siderophore systems (aerobactin, salmochelin, colistin) that enable iron scavenging. This phenotype is of particular concern given the recent convergence of hypervirulent with multidrug resistant isolates. Whilst we note hypervirulent isolates present (defined here as kleborate virulence score > 1, i.e. encoding virulence factors in addition to the often chromosomal yersiniabactin) from the very early sampling times on in our collection (Fig. S11A,B, Fig. S5A, Fig. S6A, Fig. S7A), these remain sporadic; in contrast to multidrug-resistant isolates (Fig. S11C,D) are absent at these early sampling times but rapidly become the majority of isolates from 2007/8 onwards (Fig. S11D). Whilst we note a total number of > 10 isolates for both ST86 and ST268, two STs carrying the <emph>rmpA2</emph> gene and widely observed as hypervirulent phenotypes, these occur in different years and wards, highlighting that hospital-derived transmission is highly unlikely (Fig. S11B). Whilst it is of concern that we observe some of these potentially hypervirulent isolates (encoding for <emph>rmpA2</emph>) also encoding an ESBL gene (22 total; 9 of these ST86, no ST268) there seems to be no selective advantage; the first isolate carrying this combination is observed in 2010 but a maximum of 4 isolates per year are observed until 2020. We also noted one <emph>K. variicola</emph> isolate with predicted virulence genes; investigation using long-read sequencing confirms that the operon is present on the IncFIB/IncFII plasmid (Fig. S12). This strain also carries an IncFY plasmid, again highlighting that whilst represented in lower numbers, these species can readily acquire the same plasmids as <emph>K. pneumoniae</emph>. This small number of putative hypervirulent isolates is in accordance not only with bloodstream isolates in general but also with a study comparing carriage with infectious isolates from adults in Blantyre, where only a very small number of isolates encoding virulence determinants were identified, all of them in infectious isolates [[<reflink idref="bib68" id="ref84">68</reflink>]].</p> <hd id="AN0177062508-17">Discussion</hd> <p>Neonatal sepsis is responsible for a high proportion of deaths in children under 5 in sSA, with <emph>Klebsiella</emph> by now being both the most prevalent gram-negative pathogen and increasingly hard to treat. We have analysed a longitudinal collection of routine diagnostics invasive (bloodstream and CSF) isolates of <emph>Kpn</emph> spanning from 1998 to 2020, during which time AMR increased drastically. We determined longitudinal patterns of patient demographics, <emph>Kpn</emph> population trends, vaccine targets, resistance elements and remaining treatment options. This exceptional resource, not only for sSA but also world-wide, enables us to understand the dynamics of <emph>Kpn</emph> over an exceptionally long time scale where the sampling was not biased towards resistance types. In particular, it allows us to see the dynamics before, during, and after the significant expansion of ESBL producing <emph>Kpn</emph> at maximum resolution; and highlights the need to consider not only major types based on total numbers but the temporal dynamics of vaccine targets such as cell surface polysaccharides.</p> <p> <emph>Kpn</emph> initially showed a bimodal age distribution in patients at QECH [[<reflink idref="bib3" id="ref85">3</reflink>]] and there were concerns that increasing life expectancy would lead to a higher number of adult infections; however, this trend has yet to be seen, and adult bloodstream infections were significantly reduced overall following the widespread distribution of antiretroviral therapy [[<reflink idref="bib70" id="ref86">70</reflink>]]. The stark increase in infections from 2016 onwards is almost exclusively seen in neonates and children where ward-restricted clonal expansions that are subsequently replaced with other successful clones. This suggests that context appropriate, effective, scalable infection prevention and control (IPC) targeted towards the most vulnerable patient groups in the hospital and a better understanding of <emph>Kpn</emph> transmission routes and reservoirs would prevent a large proportion of the cases caused by <emph>Klebsiella</emph> bloodstream infection. Such approaches have the potential to protect vulnerable babies against all healthcare-associated infections rather than just one pathogen and are available now, unlike a vaccine strategy that will take years to develop and implement without guarantee for success.</p> <p>The main sequence types in our study were ST39, ST14 and ST15. Whilst ST39 and ST14 are not necessarily the most prevalent ones observed in published data from other regions [[<reflink idref="bib5" id="ref87">5</reflink>]], ST15 is widely reported in SouthEast Asia [[<reflink idref="bib10" id="ref88">10</reflink>]] causing clonal outbreaks whereas it showed a different pattern in our setting driving smaller expansions but also as a lot of sporadic isolates occurring over time. This highlights the need for more data from sSA to understand the complex diversity of this organism in high-risk areas, as not only the ST composition, but also their patterns, differ between settings. The two key insights we gain from the population pattern is that the lineages continue to replace each other as the dominant clone over longer time periods, and that in our setting, this expansion is mainly driven by an increase in cases in neonatal patients. Most of the major sequence types are present at a steady, much lower proportion, before and after their respective clonal expansions, emphasising the relevance of understanding what leads to the wave-like pattern of clonal expansions in <emph>Kpn</emph> populations. Our previous work [[<reflink idref="bib68" id="ref89">68</reflink>]] on <emph>Kpn</emph> carriage and acquisitions in adults, in the hospital and in the community, showed similar STs present in community and hospital, but hospital infections to be biased towards a few STs. Introduction of the strains causing neonatal infections into the hospital could be through healthcare personnel, medical devices, parents or caretakers; and some of these STs then proceed to spread successfully in the hospital.</p> <p>We have previously described the prolonged impact of ceftriaxone on selection for ESBL-Enterobacterales, including <emph>Kpn</emph> in adult patients in our setting, and there is a need to strengthen antimicrobial stewardship to reduce unnecessary use of this agent alongside IPC interventions [[<reflink idref="bib68" id="ref90">68</reflink>]]. The temporal <emph>Kpn</emph> variability also favours prioritisation of IPC strategies to prevent the introduction of new lineages. It may be that increased IPC efforts in response to ward outbreaks contribute to or are the single cause for the sudden stops of clonal expansions. We have little understanding of the ability of <emph>Kpn</emph> to survive on abiotic surfaces, which represents one of the key differences between HIC and LMIC settings, as the latter often use wooden furniture and bristled plastic tubing due to limited resources, which might lead to different <emph>Kpn</emph> lineages being successful. Recent work on dry surface biofilms [[<reflink idref="bib71" id="ref91">71</reflink>]] has shown that the respective strain was able to survive over prolonged periods, further highlighting our need for a better understanding of biofilm formation and persistence on different hospital materials and how appropriate recommended IPC measures are for current clinical clones. A similar question remains open regarding agents used for disinfection, as resource-limited settings often rely on basic soap agents and use unsafe water, whilst HIC routinely use industrially produced, quality-controlled disinfectants.</p> <p>Our data allow insight into AMR patterns in our setting. Ceftriaxone resistance, almost exclusively due to the ESBL-encoding <emph>bla</emph><subs>CTX-M-15</subs>, is widespread. Whilst carbapenems are rarely available and resistance is already emerging [[<reflink idref="bib72" id="ref92">72</reflink>]], several treatment options are still viable, such as amikacin. Our analysis also emphasises again that aminoglycoside resistance is notoriously hard to predict and might be impacted by the genetic background (for example efflux pumps) and not only acquired resistance genes, and should therefore at this stage not be ruled out based on genomic data but confirmed via phenotyping. Most isolates were phenotypically resistant to gentamicin; whether this is consequent on the predicted resistance genes, or whether other mechanisms drive this resistance is important to determine to improve the value of genotypic predictions for aminoglycosides. It will remain to be further investigated whether this is due to other interfering genes, low expression of the resistance gene, or heteroresistance in the population; which needs to be carefully considered [[<reflink idref="bib74" id="ref93">74</reflink>]]. We furthermore observe a trend in re-emergence of chloramphenicol sensitivity, putatively in part driven by the spread of a non-functional CAT copy (CatB4) [[<reflink idref="bib57" id="ref94">57</reflink>]]. Continuing to monitor re-emergence of susceptibility to agents no longer in use is of high relevance to allow timely re-introduction of drugs and can give us insights into the potential of rotating antimicrobial regiments over longer time spans to allow sensitivity to re-emerge.</p> <p>There was little resistance predicted and observed to colistin, a last-line treatment option, in QECH <emph>Kpn</emph> isolates. Especially the lack of mobile resistance elements is encouraging, but also stresses the urgent need for better management of animal health as colistin is widely used, knowingly and as part of food products farmers are not necessarily aware of, in the increasing mid-size farming industry [[<reflink idref="bib75" id="ref95">75</reflink>]]. Another exciting new development are initial results of combining fosfomycin and flomoxef [[<reflink idref="bib76" id="ref96">76</reflink>]]; we observe only sporadic fosfomycin resistance mechanisms and very little spread of <emph>ampC</emph> genes, the only non-carbapenemases that would deactivate flomoxef, although we note that there are also counterindications for fosfomycin use in invasive infections.</p> <p>As extensively resistant bacterial pathogens emerge and spread, there is an increasing urgency to develop vaccines to break the arms race between new antimicrobials and new resistance mechanisms. In this case, there is interest in developing maternally administered <emph>Klebsiella</emph> vaccines to prevent neonatal sepsis. A target of high interest is the extracellular polysaccharides that can be typed based on their respective operons, which are assigned different K-types (capsular antigen) and O-types (the lipopolysaccharide O-antigen chain). The K-antigen shows a very high diversity; and whilst we can identify several dominant types in our study, the long timeframe of our study highlights the importance of considering the dynamic nature of K-antigens when considering vaccine design. Here, temporal fluctuations detected just in Blantyre strongly suggest that a K-type vaccine would become ineffective very rapidly given the frequent changes in dominant K-type, which might be driven by phage pressure in the environment and thus highly unpredictable. It is also unclear if successful vaccination against the major polysaccharide types would lead to a degree of cross-protection against non-targeted types or whether lack of such cross-protection would facilitate rapid emergence of infections caused by vaccine escape lineages.</p> <p>In contrast, there are far fewer O-antigen types, making this a more attractive target. Recent efforts have compared several large datasets with O- and K-typing either determined by PCR or WGS [[<reflink idref="bib77" id="ref97">77</reflink>]]; and identified that a conjugate vaccine targeting O1v1/2 (~ serotype O1) [[<reflink idref="bib26" id="ref98">26</reflink>]], O2v2 (~ serotype O2afg) [[<reflink idref="bib26" id="ref99">26</reflink>]] and O3b (~ serotype O3b) [[<reflink idref="bib26" id="ref100">26</reflink>]] has the potential to prevent the majority (> 70%) of cases of <emph>Kpn</emph>-BSI. For the most at-risk population in our setting, the neonates, this would cover 87% of cases (338/388) assuming O1a and O1b were included into O1 and no other subtypes were discovered [[<reflink idref="bib29" id="ref101">29</reflink>]]; importantly it would however not have prevented the ST340 outbreak in the neonatal unit previously described [[<reflink idref="bib19" id="ref102">19</reflink>]] (O4). It thus seems of relevance to consider including the currently low-abundance O-antigens as well, in particular given the interchangeability for O-types within the same STs. Reports from other highly understudied settings also report other main O-types in hospital isolates, e.g. O5 in a study in Pakistan [[<reflink idref="bib52" id="ref103">52</reflink>], [<reflink idref="bib78" id="ref104">78</reflink>]] or a diversity of O-types in the Caribbean where this O-Ag vaccine would only cover 62% (162/260) [[<reflink idref="bib79" id="ref105">79</reflink>]]. Selection pressure might not directly drive evolution given that <emph>Kpn</emph> is not human restricted but could likely select for strains with currently less abundant O-types to increase in prevalence. Further, there remains an open question of how accessible the O-antigens are to antibodies, which a recent study calls into question [[<reflink idref="bib80" id="ref106">80</reflink>]]; we currently have very limited understanding what drives the extent of capsule expression per cell in non-hypermucoid isolates and how different stages of the infection impact this. It is known from in vitro studies that capsule expression even in the same experimental setting can differ widely within clonal lineages, which also impacts the bacterial ability to escape the complement system [[<reflink idref="bib81" id="ref107">81</reflink>]].</p> <p>Our data, and the recent identification of a novel form of the long-studied O1 antigen type [[<reflink idref="bib29" id="ref108">29</reflink>], [<reflink idref="bib82" id="ref109">82</reflink>]–[<reflink idref="bib84" id="ref110">84</reflink>]], highlights that we have very little understanding of the relevance of the O- and K-types on the success of <emph>Kpn</emph> lineages, whether any additional genes on the chromosomal backbone can further modify the structures and lead to different serotypes with the same O-locus, as well as whether any O-/K-combinations are more or less favourable. Closing such knowledge gaps will be central to design of vaccines as we also have little understanding for the propensity of sequence type combinations with specific vaccine target serotypes such as capsule or O-antigen. A more solid understanding of O-/K-type variations, both regarding their biochemistry and whether they contribute to epidemiological success, is crucial given the high interest to use these as targets for maternally administered vaccines against neonatal sepsis.</p> <p>This highlights that we have very little understanding of the relevance of the O- and K-types on the success of Kpn lineages, whether any additional genes on the chromosomal backbone can further modify the structures and lead to different serotypes with the same O-locus, as well as whether any O-/K-combinations are more or less favourable. A more solid understanding of O-/K-type variations, both regarding their biochemistry and whether they contribute to epidemiological success, is crucial given the high interest to use these as targets for maternally administered vaccines against neonatal sepsis.</p> <hd id="AN0177062508-18">Conclusions</hd> <p>In summary, our study provides a unique insight into long-term patterns of <emph>Kpn</emph> hospital isolates from a low-income setting, spanning across a time period where the number of MDR and ESBL-producing infections increased rapidly across the globe. We show that whilst STs known as high-risk in other settings are common, these do not always show similar success in different settings; and we observe a significant number of isolates from otherwise rarely observed STs, causing hospital infections. This again emphasises the importance of including the relevant settings into studies aiming to identify treatment or vaccine targets as we currently have a poor understanding of what makes an ST a high-risk lineage in each setting at a given time. Our data also emphasises the urgent need to consider temporal variation when considering treatment or vaccine targets, as some of the main vaccine targets show strong temporal fluctuations; the most prevalent type now circulating is unlikely to cause the next outbreak. The close relatedness of isolates from the same ward in the same time frame strongly indicates that the majority of infections are hospital-acquired, so whilst vaccine development and alternative treatment options are highly relevant; at this stage, focused efforts to improve IPC are key to reducing <emph>Kpn</emph> disease burden now [[<reflink idref="bib56" id="ref111">56</reflink>]].</p> <hd id="AN0177062508-19">Acknowledgements</hd> <p>We would like to thank the Sanger Institute Pathogen Genomics group for expert computational support. We acknowledge and thank all the clinical staff and patients at QECH over the years for their contribution; and the MLW core microbiology team for expert support in storage and handling of the isolates.</p> <hd id="AN0177062508-20">Authors' contributions</hd> <p>Conceptualization: EH, NAF; methodology: EH, NAF; software: EH, JML; validation: EH, OP, AZ; formal analysis: EH, OP; investigation: EH, OP, AZ, PM, PS, ET, FG, RL, SL, JC, JML; resources: SB, PS, ET, KK, NRT, NAF; data curation: EH, OP, PM, RL, JC, JML, AZ; writing—original draft: EH, OP, NAF; writing—review and editing: all authors; visualisation: EH, OP, JML, NAF; supervision: EH, CM, SL, KK, NAF; project administration: EH, NAF; funding acquisition: EH, NRT, NAF. All authors read and approved the final manuscript.</p> <hd id="AN0177062508-21">Funding</hd> <p>We acknowledge funding from Wellcome (EH; grant 217303/Z/19/Z) and the BMGF (NAF and EH; grant INV-005180) as well as Wellcome funding providing core support for the Wellcome Sanger Institute (206194) and MLW (206454). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</p> <hd id="AN0177062508-22">Availability of data and materials</hd> <p>All sequence data is available on SRA/ENA under BioProject PRJEB42462 https://<ulink href="http://www.ebi.ac.uk/ena/browser/view/PRJEB42462">www.ebi.ac.uk/ena/browser/view/PRJEB42462</ulink> (short read data) and PRJNA1102521 https://<ulink href="http://www.ncbi.nlm.nih.gov/bioproject/PRJNA1102521">www.ncbi.nlm.nih.gov/bioproject/PRJNA1102521</ulink> (long-read data); specific accessions and all additional data is provided in the supporting tables S1-S6. All data and code used to create the analyses and is available as R package QECHospitalKlebs v0.0.9 (https://github.com/EvitaH/QECHospitalKlebs; https://doi.org/10.5281/zenodo.8421658).</p> <hd id="AN0177062508-23">Declarations</hd> <p></p> <hd id="AN0177062508-24">Ethics approval and consent to participate</hd> <p>The <emph>Klebsiella</emph> Isolates sequenced for this study were originally generated by the routine clinical diagnostic microbiology service at QECH, therefore individual level consent was not required. Ethical approval for this study was granted by the University of Malawi College of Medicine Research Ethics Committee (COMREC) (P.11/18/2541) and isolates were shipped under a Nagoya Protocol compliant Access and Benefit Sharing agreement between the Government of Malawi and the Wellcome Sanger Institute. The research conformed to the principle of the Helsinki Declaration.</p> <hd id="AN0177062508-25">Consent for publication</hd> <p>Not applicable.</p> <hd id="AN0177062508-26">Competing interests</hd> <p>The authors declare that they have no competing interests.</p> <hd id="AN0177062508-27">Supplementary Information</hd> <p>Graph: Additional file 1: Supplementary figures Fig.S1-Fig.S12 and respective figure legends and a description of Tables S1-S6.</p> <p>Graph: Additional file 2: Supplementary tables Table S1-Table S6 as worksheets in one xls file.</p> <hd id="AN0177062508-28">Publisher's Note</hd> <p>Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.</p> <ref id="AN0177062508-29"> <title> References </title> <blist> <bibl id="bib1" idref="ref1" type="bt">1</bibl> <bibtext> Liu L, Oza S, Hogan D, Chu Y, Perin J, Zhu J. 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  Data: Longitudinal analysis within one hospital in sub-Saharan Africa over 20 years reveals repeated replacements of dominant clones of Klebsiella pneumoniae and stresses the importance to include temporal patterns for vaccine design considerations
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  Data: <searchLink fieldCode="AR" term="%22Eva+Heinz%22">Eva Heinz</searchLink><br /><searchLink fieldCode="AR" term="%22Oliver+Pearse%22">Oliver Pearse</searchLink><br /><searchLink fieldCode="AR" term="%22Allan+Zuza%22">Allan Zuza</searchLink><br /><searchLink fieldCode="AR" term="%22Sithembile+Bilima%22">Sithembile Bilima</searchLink><br /><searchLink fieldCode="AR" term="%22Chisomo+Msefula%22">Chisomo Msefula</searchLink><br /><searchLink fieldCode="AR" term="%22Patrick+Musicha%22">Patrick Musicha</searchLink><br /><searchLink fieldCode="AR" term="%22Patriciah+Siyabu%22">Patriciah Siyabu</searchLink><br /><searchLink fieldCode="AR" term="%22Edith+Tewesa%22">Edith Tewesa</searchLink><br /><searchLink fieldCode="AR" term="%22Fabrice+E%2E+Graf%22">Fabrice E. Graf</searchLink><br /><searchLink fieldCode="AR" term="%22Rebecca+Lester%22">Rebecca Lester</searchLink><br /><searchLink fieldCode="AR" term="%22Samantha+Lissauer%22">Samantha Lissauer</searchLink><br /><searchLink fieldCode="AR" term="%22Jennifer+Cornick%22">Jennifer Cornick</searchLink><br /><searchLink fieldCode="AR" term="%22Joseph+M%2E+Lewis%22">Joseph M. Lewis</searchLink><br /><searchLink fieldCode="AR" term="%22Kondwani+Kawaza%22">Kondwani Kawaza</searchLink><br /><searchLink fieldCode="AR" term="%22Nicholas+R%2E+Thomson%22">Nicholas R. Thomson</searchLink><br /><searchLink fieldCode="AR" term="%22Nicholas+A%2E+Feasey%22">Nicholas A. Feasey</searchLink>
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  Data: Genome Medicine, Vol 16, Iss 1, Pp 1-17 (2024)
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  Group: HoldingsInfo
  Data: LCC:Medicine<br />LCC:Genetics
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22AMR%22">AMR</searchLink><br /><searchLink fieldCode="DE" term="%22Malawi%22">Malawi</searchLink><br /><searchLink fieldCode="DE" term="%22Genome+surveillance%22">Genome surveillance</searchLink><br /><searchLink fieldCode="DE" term="%22Healthcare-associated%22">Healthcare-associated</searchLink><br /><searchLink fieldCode="DE" term="%22Nosocomial+infections%22">Nosocomial infections</searchLink><br /><searchLink fieldCode="DE" term="%22Surface+antigens%22">Surface antigens</searchLink><br /><searchLink fieldCode="DE" term="%22Medicine%22">Medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Genetics%22">Genetics</searchLink><br /><searchLink fieldCode="DE" term="%22QH426-470%22">QH426-470</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Abstract Background Infections caused by multidrug-resistant gram-negative bacteria present a severe threat to global public health. The WHO defines drug-resistant Klebsiella pneumoniae as a priority pathogen for which alternative treatments are needed given the limited treatment options and the rapid acquisition of novel resistance mechanisms by this species. Longitudinal descriptions of genomic epidemiology of Klebsiella pneumoniae can inform management strategies but data from sub-Saharan Africa are lacking. Methods We present a longitudinal analysis of all invasive K. pneumoniae isolates from a single hospital in Blantyre, Malawi, southern Africa, from 1998 to 2020, combining clinical data with genome sequence analysis of the isolates. Results We show that after a dramatic increase in the number of infections from 2016 K. pneumoniae becomes hyperendemic, driven by an increase in neonatal infections. Genomic data show repeated waves of clonal expansion of different, often ward-restricted, lineages, suggestive of hospital-associated transmission. We describe temporal trends in resistance and surface antigens, of relevance for vaccine development. Conclusions Our data highlight a clear need for new interventions to prevent rather than treat K. pneumoniae infections in our setting. Whilst one option may be a vaccine, the majority of cases could be avoided by an increased focus on and investment in infection prevention and control measures, which would reduce all healthcare-associated infections and not just one.
– Name: TypeDocument
  Label: Document Type
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  Data: article
– Name: Format
  Label: File Description
  Group: SrcInfo
  Data: electronic resource
– Name: Language
  Label: Language
  Group: Lang
  Data: English
– Name: ISSN
  Label: ISSN
  Group: ISSN
  Data: 1756-994X
– Name: NoteTitleSource
  Label: Relation
  Group: SrcInfo
  Data: https://doaj.org/toc/1756-994X
– Name: DOI
  Label: DOI
  Group: ID
  Data: 10.1186/s13073-024-01342-3
– Name: URL
  Label: Access URL
  Group: URL
  Data: <link linkTarget="URL" linkTerm="https://doaj.org/article/389b70344adb4c468575cb18c6a2d66d" linkWindow="_blank">https://doaj.org/article/389b70344adb4c468575cb18c6a2d66d</link>
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  Label: Accession Number
  Group: ID
  Data: edsdoj.389b70344adb4c468575cb18c6a2d66d
PLink https://login.libproxy.scu.edu/login?url=https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsdoj&AN=edsdoj.389b70344adb4c468575cb18c6a2d66d
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1186/s13073-024-01342-3
    Languages:
      – Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 17
        StartPage: 1
    Subjects:
      – SubjectFull: AMR
        Type: general
      – SubjectFull: Malawi
        Type: general
      – SubjectFull: Genome surveillance
        Type: general
      – SubjectFull: Healthcare-associated
        Type: general
      – SubjectFull: Nosocomial infections
        Type: general
      – SubjectFull: Surface antigens
        Type: general
      – SubjectFull: Medicine
        Type: general
      – SubjectFull: Genetics
        Type: general
      – SubjectFull: QH426-470
        Type: general
    Titles:
      – TitleFull: Longitudinal analysis within one hospital in sub-Saharan Africa over 20 years reveals repeated replacements of dominant clones of Klebsiella pneumoniae and stresses the importance to include temporal patterns for vaccine design considerations
        Type: main
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            NameFull: Eva Heinz
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            NameFull: Oliver Pearse
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            NameFull: Fabrice E. Graf
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            NameFull: Rebecca Lester
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            NameFull: Nicholas R. Thomson
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          Dates:
            – D: 01
              M: 05
              Type: published
              Y: 2024
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              Value: 1756994X
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              Value: 16
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            – TitleFull: Genome Medicine
              Type: main
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