Bibliographic Details
| Title: |
Separation and identification of ceramides derived from human plasma sphingomyelins |
| Authors: |
Bengt Samuelsson, Karin Samuelsson |
| Source: |
Journal of Lipid Research, Vol 10, Iss 1, Pp 47-55 (1969) |
| Publisher Information: |
Elsevier, 1969. |
| Publication Year: |
1969 |
| Collection: |
LCC:Biochemistry |
| Subject Terms: |
phospholipase C, ceramide acetate, argentation chromatography, trimethylsilyl ethers, gas-liquid chromatography, mass spectrometry, Biochemistry, QD415-436 |
| More Details: |
Sphingomyelins from human blood plasma have been converted into ceramides by enzymatic hydrolysis with phospholipase C. After acetylation the ceramides were fractionated by thin-layer chromatography on silica gel containing silver nitrate. Four main fractions obtained by this method were subsequently converted to di-O-trimethylsilyl ether derivatives and separated by gas-liquid chromatography on 1% OV-1. 2-11 components could be distinguished in each of the four fractions. The major fractions emerging from the gas chromatograph were analyzed by mass spectrometry and their main molecular species were identified. Two of the gas chromatographic fractions contained essentially pure molecular species, namely N-tetracosenoyl sphingosine and N-tetracosenoylsphinga-4,14-dienine. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
0022-2275 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S0022227520426483; https://doaj.org/toc/0022-2275 |
| DOI: |
10.1016/S0022-2275(20)42648-3 |
| Access URL: |
https://doaj.org/article/37a1eefa831944fb9d5ca04ab6eb7381 |
| Accession Number: |
edsdoj.37a1eefa831944fb9d5ca04ab6eb7381 |
| Database: |
Directory of Open Access Journals |
