| Title: |
The diagnostic value of next-generation sequencing technology in sepsis |
| Authors: |
Xiao-guang Cao, Shu-sheng Zhou, Chun-yan Wang, Kui Jin, Hua-dong Meng |
| Source: |
Frontiers in Cellular and Infection Microbiology, Vol 12 (2022) |
| Publisher Information: |
Frontiers Media S.A., 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Microbiology |
| Subject Terms: |
Next-generation sequencing, sepsis, pathogen, diagnosis, ICU, Microbiology, QR1-502 |
| More Details: |
ObjectiveThis study aims to assess the clinical utility of next-generation sequencing (NGS) in sepsis diagnosis.MethodsA prospective study was conducted on patients with a high suspicion of sepsis by unknown pathogens from January 2017 to December 2021. Blood samples were taken from patients to perform NGS, blood culture (BC), leucocyte (WBC), procalcitonin (PCT), creatinine (CREA), Albumin (ALB) and C-reactive protein (CRP) tests.ResultsThe feedback time for BC was 3~5 days for bacteria and 5~7 days for fungi, while the turnover time for NGS was only 24 h. The clinical diagnosis was considered the “gold standard”. 83 patients passed our inclusion criteria and were separated into two groups by clinical diagnosis. 62 met the clinical diagnosis criteria for sepsis and 21 were non-sepsis. The data from the two groups were retrospectively compared and analyzed. Of 62 sepsis in 83 patients, 8(9.64%) were diagnosed by both BC and NGS, 51 (61.45%) by NGS only, 1(1.20%) by BC and 2 (2.41%) by conventional testing only; PCT, CREA, CRP levels and the detection rate of NGS and BC were higher in the sepsis group than in the non-sepsis group, while ALB levels were lower (p |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2235-2988 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fcimb.2022.899508/full; https://doaj.org/toc/2235-2988 |
| DOI: |
10.3389/fcimb.2022.899508 |
| Access URL: |
https://doaj.org/article/378c5975b6454f23ba653903f9d59d3f |
| Accession Number: |
edsdoj.378c5975b6454f23ba653903f9d59d3f |
| Database: |
Directory of Open Access Journals |
