Bibliographic Details
| Title: |
Activity-dependent labeling and manipulation of fentanyl-recruited striatal ensembles using ArcTRAP approach |
| Authors: |
Xueyi Xie, Ruifeng Chen, Xuehua Wang, Laura Smith, Jun Wang |
| Source: |
STAR Protocols, Vol 4, Iss 3, Pp 102369- (2023) |
| Publisher Information: |
Elsevier, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Science (General) |
| Subject Terms: |
Neuroscience, Behavior, Science (General), Q1-390 |
| More Details: |
Summary: Understanding the memory substrates underlying substance abuse requires the permanent tagging and manipulation of drug-recruited neural ensembles. Here, we present a protocol for activity-dependent labeling and chemogenetic manipulation of fentanyl-activated striatal ensembles using the ArcTRAP approach. We outline the necessary steps to breed ArcTRAP mice, prepare drugs and reagents, conduct behavioral training, and perform tagging and manipulation. This approach can be adapted to investigate drug-recruited ensembles in other brain regions, providing a versatile tool for exploring the neural mechanisms underlying addiction.For complete details on the use and execution of this protocol, please refer to Wang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2666-1667 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666166723003362; https://doaj.org/toc/2666-1667 |
| DOI: |
10.1016/j.xpro.2023.102369 |
| Access URL: |
https://doaj.org/article/371249464eff4ee1bef46929c446a6f7 |
| Accession Number: |
edsdoj.371249464eff4ee1bef46929c446a6f7 |
| Database: |
Directory of Open Access Journals |
