| Title: |
A Method for Multiple Mycotoxin Analysis in Wines by Solid Phase Extraction and Multifunctional Cartridge Purification, and Ultra-High-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry |
| Authors: |
Naoki Mochizuki, Masayoshi Tamura, Atsuo Uyama, Ayumi Takahashi |
| Source: |
Toxins, Vol 4, Iss 6, Pp 476-486 (2012) |
| Publisher Information: |
MDPI AG, 2012. |
| Publication Year: |
2012 |
| Collection: |
LCC:Medicine |
| Subject Terms: |
UHPLC-MS/MS, mycotoxins, wine, solid phase extraction, multiple analyses, Medicine |
| More Details: |
An analytical method using two solid phase extractions and ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed for the identification and quantification of 14 mycotoxins (patulin, deoxynivalenol, aflatoxins B1, B2, G1, G2, M1, T-2 toxin, HT-2 toxin, zearalenone, fumonisins B1, B2, B3, and ochratoxin A) in domestic and imported wines. Mycotoxins were purified with an Oasis HLB cartridge, followed by a MultiSepTM #229 Ochra. As a result, sufficient removal of the pigments and highly polar matrices from the red wines was achieved. UHPLC conditions were optimized, and 14 mycotoxins were separated in a total of 13 min. Determinations performed using this method produced high correlation coefficients for the 14 mycotoxins (R > 0.990) and recovery rates ranging from 76 to 105% with good repeatability (relative standard deviation RSD < 12%). Twenty-seven samples of domestic and imported wines were analyzed using this method. Although ochratoxin A (OTA) and fumonisins (FMs) were detected in several samples, the FM levels were less than limits of quantification (LOQs) (1 μg/L), and even the largest of the OTA levels was below the EU regulatory level (2 μg/L). These results suggest that the health risk posed to consumers from the wines available in Japan is relatively low. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2072-6651 |
| Relation: |
http://www.mdpi.com/2072-6651/4/6/476; https://doaj.org/toc/2072-6651 |
| DOI: |
10.3390/toxins4060476 |
| Access URL: |
https://doaj.org/article/da36e77ea7be449f8ce3c87a7cb4be00 |
| Accession Number: |
edsdoj.36e77ea7be449f8ce3c87a7cb4be00 |
| Database: |
Directory of Open Access Journals |
