Bibliographic Details
| Title: |
Functional maturation of human iPSC-derived pyramidal neurons in vivo is dependent on proximity with the host tissue |
| Authors: |
Célia Raïs, Daniela Gaspar Santos, Giulia Sansone, Stéphane Blanchard, Jean-Pierre Bourgeois, Bernd Jagla, Baptiste Saudemont, Laurène Schlick, Stéphanie Pons, Uwe Maskos |
| Source: |
Frontiers in Cellular Neuroscience, Vol 17 (2023) |
| Publisher Information: |
Frontiers Media S.A., 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Neurosciences. Biological psychiatry. Neuropsychiatry |
| Subject Terms: |
human induced pluripotent stem cells, neural precursor cells, grafting, neurodevelopment, electron microscopy, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571 |
| More Details: |
Human induced pluripotent stem cells (hiPSCs) have been used extensively in vitro to model early events in neurodevelopment. Because of a number of shortcomings, previous work has established a potential to use these cells in vivo after transplantation into the mouse brain. Here, we describe a systematic approach for the analysis of transplanted hiPSC-derived neurons and glial cells over time in the mouse brain. Using functional two-photon imaging of GCaMP6f- expressing human neural cells, we define and quantify the embryonic-like features of their spontaneous activity. This is substantiated by detailed electron microscopy (EM) of the graft. We relate this to the synaptic development the neurons undergo up to 7 months in vivo. This system can now be used further for the genetic or experimental manipulation of developing hiPSC-derived cells addressing neurodevelopmental diseases like schizophrenia or Autism Spectrum Disorder. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1662-5102 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fncel.2023.1259712/full; https://doaj.org/toc/1662-5102 |
| DOI: |
10.3389/fncel.2023.1259712 |
| Access URL: |
https://doaj.org/article/e36c19e1b9ef41d1a6d6d7bc646fb362 |
| Accession Number: |
edsdoj.36c19e1b9ef41d1a6d6d7bc646fb362 |
| Database: |
Directory of Open Access Journals |
