Effect of Pien Tze Huang on Lymphangiogenesis Mediated by LncRNA-ANRIL in Colorectal Cancer
| Title: | Effect of Pien Tze Huang on Lymphangiogenesis Mediated by LncRNA-ANRIL in Colorectal Cancer |
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| Authors: | Yao LU, Min ZHANG, Jie LIU, Qianqian MAO, Zhiyun CAO, Jiumao LIN |
| Source: | 康复学报, Vol 30, Pp 387-394 (2020) |
| Publisher Information: | Editorial Office of Rehabilitation Medicine, 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Medicine |
| Subject Terms: | colorectal cancer, Pien Tze Huang, human lymphatic endothelial cells, LncRNA-ANRIL, lymphangiogenesis, Medicine |
| More Details: | Objective:To evaluate the inhibitory effect and mechanism of Pien Tze Huang (PZH) on long non-coding RNA-ANRIL(LncRNA-ANRIL) mediated lymphangiogenesis in colorectal cancer (CRC) in vitro.Methods:HCT-116 cells were cultured in vitro, and divided into control group and PZH group (treated with 0.25, 0.50, 0.75 mg/mL). CCK-8 assay was used to evaluate cell ability. The expression of LncRNA-ANRIL was detected by RT-qPCR, and the expression of VEGF-C was examined by Western blot. HCT-116 cells were transfected with Lipofectamine RNAiMAX and divided into Si-NC and Si-ANRIL groups. RT-qPCR was used to detect the expression of LncRNA-ANRIL. Phase-contrast microscope was used to observe the cell morphology. CCK-8 assay was used to detect cell viability. Western blot was used to detect the expression of VEGF-C protein. Treat HCT-116 cells with Si-NC, Si-ANRIL and Si-NC+PZH(0.25 mg/mL) respectively and collect cell culture supernatants (Tumor culture supernatants, TSNs) for theculture of human lymphatic endothelium cell (HLEC). CCK-8 assay was used to detect cell proliferation, cell migration and invasion were evaluated by Transwell assay. Tube Formation assay was used to observe lymphatic vessel formation ability.Results:Treatment of HCT-116 cells with different doses of PZH significantly inhibited the viability (P< 0.01). At the same time, PZH treatment in HCT-116 cells also reduced the expression of LncRNA-ANRIL(P< 0.01) and the expression of VEGF-C protein in a dose-dependent. Silencing LncRNA-ANRIL in HCT-116 cells, compared with Si-NC group, the expression of LncRNA-ANRIL in Si-ANRIL group was significantly reduced (P< 0.05), and the expression of cell density, cell viability (P< 0.01) and VEGF-C protein was also decreased. After the collected TSNssupernatant was used to treat HLEC cells, compared with the Si-NC group, Si-ANRIL and Si-NC+PZH(0.25 mg/mL) groups could remarkably decrease HLEC cell viability, migration and invasion ability and tube formation ability (P< 0.01).Conclusion:PZH can inhibit the expression of LncRNA-ANRIL in colorectal cancer cells and its mediated lymphangiogenesis in colorectal cancer. LncRNA-ANRIL is one of the targets of PZH in inhibiting lymphangiogenesis in colorectal cancer. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English Chinese |
| ISSN: | 2096-0328 |
| Relation: | http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2020.05008; https://doaj.org/toc/2096-0328 |
| DOI: | 10.3724/SP.J.1329.2020.05008 |
| Access URL: | https://doaj.org/article/35f2fa22c5174208bc0afd9c0fbbac96 |
| Accession Number: | edsdoj.35f2fa22c5174208bc0afd9c0fbbac96 |
| Database: | Directory of Open Access Journals |
| ISSN: | 20960328 |
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| DOI: | 10.3724/SP.J.1329.2020.05008 |
| Published in: | 康复学报 |
| Language: | English Chinese |