Academic Journal
Mechanism by which Rab5 promotes regeneration and functional recovery of zebrafish Mauthner axons
| Title: | Mechanism by which Rab5 promotes regeneration and functional recovery of zebrafish Mauthner axons |
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| Authors: | Jiantao Cui, Yueru Shen, Zheng Song, Dinggang Fan, Bing Hu |
| Source: | Neural Regeneration Research, Vol 20, Iss 6, Pp 1816-1824 (2025) |
| Publisher Information: | Wolters Kluwer Medknow Publications, 2025. |
| Publication Year: | 2025 |
| Collection: | LCC:Neurology. Diseases of the nervous system |
| Subject Terms: | axonal regeneration, mauthner cell, nerve regeneration, rab5, zebrafish, Neurology. Diseases of the nervous system, RC346-429 |
| More Details: | Rab5 is a GTPase protein that is involved in intracellular membrane trafficking. It functions by binding to various effector proteins and regulating cellular responses, including the formation of transport vesicles and their fusion with the cellular membrane. Rab5 has been reported to play an important role in the development of the zebrafish embryo; however, its role in axonal regeneration in the central nervous system remains unclear. In this study, we established a zebrafish Mauthner cell model of axonal injury using single-cell electroporation and two-photon axotomy techniques. We found that overexpression of Rab5 in single Mauthner cells promoted marked axonal regeneration and increased the number of intra-axonal transport vesicles. In contrast, treatment of zebrafish larvae with the Rab kinase inhibitor CID-1067700 markedly inhibited axonal regeneration in Mauthner cells. We also found that Rab5 activated phosphatidylinositol 3-kinase (PI3K) during axonal repair of Mauthner cells and promoted the recovery of zebrafish locomotor function. Additionally, rapamycin, an inhibitor of the mechanistic target of rapamycin downstream of PI3K, markedly hindered axonal regeneration. These findings suggest that Rab5 promotes the axonal regeneration of injured zebrafish Mauthner cells by activating the PI3K signaling pathway. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1673-5374 1876-7958 |
| Relation: | https://journals.lww.com/10.4103/NRR.NRR-D-23-00529; https://doaj.org/toc/1673-5374; https://doaj.org/toc/1876-7958 |
| DOI: | 10.4103/NRR.NRR-D-23-00529 |
| Access URL: | https://doaj.org/article/35c48cd79bf04c7e971d2513608df1f1 |
| Accession Number: | edsdoj.35c48cd79bf04c7e971d2513608df1f1 |
| Database: | Directory of Open Access Journals |
| ISSN: | 16735374 18767958 |
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| DOI: | 10.4103/NRR.NRR-D-23-00529 |
| Published in: | Neural Regeneration Research |
| Language: | English |