Bibliographic Details
| Title: |
miR-215-5p Plays a Key Role in Suppressing Vascular Invasion and Recurrence in Hepatocellular Carcinoma by Blocking Vasculogenic Mimicry |
| Authors: |
Heng Zhang, Xi Lan, Liquan Cai, Xunfeng Gao, Feng Gao, Dan Yu, Jinlong Zhang, Jinhui Zhang, Qinwen Tai |
| Source: |
Frontiers in Bioscience-Elite, Vol 16, Iss 1, p 6 (2024) |
| Publisher Information: |
IMR Press, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Environmental sciences
LCC:Microbiology |
| Subject Terms: |
hepatocellular carcinoma, mir-215-5p, zeb2, vasculogenic mimicry, vascular invasion, recurrent metastasis, Environmental sciences, GE1-350, Microbiology, QR1-502 |
| More Details: |
Background: This research explores the significance of miR-215-5p and vasculogenic mimicry (VM) in forecasting the prognosis for hepatocellular carcinoma (HCC). Methods: We analyzed HCC-associated miRNA expression profiles using data from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO). Samples included tissue and blood from 80 early-stage HCC patients and serum from 120 healthy individuals. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was employed to measure miR-215-5p and zinc finger E-box binding homeobox 2 (ZEB2) gene expressions. Hematoxylin and eosin (H&E) and CD34/Periodic Acid-Schiff (PAS) double staining assessed VM presence in HCC tissue sections. Bioinformatics tools predicted interactions between miR-215-5p and ZEB2, confirmed through luciferase reporter assays. We also examined the impact of miR-215-5p or ZEB2 overexpression on HCC cell invasion, migration, and VM formation using scratch, Transwell invasion assays, and Matrigel 3D cultures. Results: Bioinformatics analysis indicated that miR-215-5p was under-expressed in HCC, particularly in cases with vascular invasion, which correlated with worse patient outcomes. In contrast, ZEB2, targeted by miR-215-5p, was overexpressed in HCC. RT-qPCR validated these expression patterns in HCC tissues. Among the HCC patients, 38 were VM positive and 42 VM negative. Logistic regression highlighted a negative correlation between miR-215-5p levels and VM positivity in HCC tissues and a positive correlation for ZEB2 with VM positivity and tumor vascular invasion. Lower miR-215-5p levels were linked to increased HCC recurrence and metastasis. Both bioinformatics analysis and luciferase assays demonstrated a direct interaction between miR-215-5p and ZEB2. Enhancing miR-215-5p levels reduced ZEB2 expression, consequently diminishing invasion, migration, and VM formation of the HCC cells in vitro. Conclusions: miR-215-5p expression inversely correlates with VM occurrence in HCC tissues, while ZEB2 expression shows a direct correlation. By targeting ZEB2, miR-215-5p may hinder VM in HCC tissues, helping to prevent vascular invasion and HCC recurrence. Thus, miR-215-5p emerges as a vital prognostic indicator for predicting vascular invasion and recurrence in HCC. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1945-0494 |
| Relation: |
https://www.imrpress.com/journal/FBE/16/1/10.31083/j.fbe1601006; https://doaj.org/toc/1945-0494 |
| DOI: |
10.31083/j.fbe1601006 |
| Access URL: |
https://doaj.org/article/35b7c3f1017045f9ae5bcc4b7355e17b |
| Accession Number: |
edsdoj.35b7c3f1017045f9ae5bcc4b7355e17b |
| Database: |
Directory of Open Access Journals |
