Bibliographic Details
| Title: |
Beneficial effects of Galectin-9 on allergen-specific sublingual immunotherapy in a Dermatophagoides farinae-induced mouse model of chronic asthma |
| Authors: |
Masaki Ikeda, Shigeki Katoh, Hiroki Shimizu, Akira Hasegawa, Katsuyo Ohashi-Doi, Mikio Oka |
| Source: |
Allergology International, Vol 66, Iss 3, Pp 432-439 (2017) |
| Publisher Information: |
Elsevier, 2017. |
| Publication Year: |
2017 |
| Collection: |
LCC:Immunologic diseases. Allergy |
| Subject Terms: |
Galectin-9, Mite allergen, Mouse, SLIT, TGF-β1, Immunologic diseases. Allergy, RC581-607 |
| More Details: |
Background: Allergen-specific sublingual immunotherapy is a potential disease-modifying treatment for allergic asthma. Galectin-9 (Gal-9), a β-galactoside-binding protein with various biologic effects, acts as an immunomodulator in excessive immunologic reactions by expanding regulatory T cells (Treg) and enhancing transforming growth factor (TGF)-β signaling. We investigated the efficacy of sublingually administered Gal-9 as an adjuvant to a specific allergen in a Dermatophagoides farinae (Df)-induced mouse model of chronic asthma. Methods: BALB/c mice were intranasally sensitized with Df extract 5 days/week for 5 weeks, and then sublingual Df-allergen extract for 2 weeks (5 days/week). Three days after the final sublingual treatment, mice were intranasally challenged with Df extract. The early asthmatic response (EAR) was evaluated 5 min after the last Df challenge. Airway hyperresponsiveness (AHR) was assayed and bronchoalveolar lavage (BAL) was performed 24 h after the last allergen challenge. Serum IgE and cytokine levels, and number of inflammatory cells in the BAL fluid (BALF) were analyzed. Results: Sublingual Df treatment in the presence of Gal-9, but not alone, significantly reduced AHR; EAR; number of eosinophils and interleukin-13 in the BALF; and serum IgE levels. BALF TGF-β1 levels were significantly increased in the presence of Gal-9 compared with Df alone. Treg depletion blocked the inhibitory effects of Gal-9 on the EAR, AHR, eosinophilic airway inflammation, and Df-specific serum IgE levels, and suppressed BALF TGF-β1 levels. Conclusions: Gal-9 exhibited beneficial effects of sublingual Df allergen-specific immunotherapy in a Df-induced mouse model of chronic asthma, possibly by Gal-9-induced TGF-β1 production in the lung. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1323-8930 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1323893016301617; https://doaj.org/toc/1323-8930 |
| DOI: |
10.1016/j.alit.2016.10.007 |
| Access URL: |
https://doaj.org/article/35ae6be3c4d445e8b8a54802ed23574d |
| Accession Number: |
edsdoj.35ae6be3c4d445e8b8a54802ed23574d |
| Database: |
Directory of Open Access Journals |
