| Title: |
Genome-Wide Gene Expression Analysis Reveals Unique Genes Signatures of Epithelial Reorganization in Primary Airway Epithelium Induced by Type-I, -II and -III Interferons |
| Authors: |
Anna Erb, Ulrich M. Zissler, Madlen Oelsner, Adam M. Chaker, Carsten B. Schmidt-Weber, Constanze A. Jakwerth |
| Source: |
Biosensors, Vol 12, Iss 11, p 929 (2022) |
| Publisher Information: |
MDPI AG, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Biotechnology |
| Subject Terms: |
microarray, gene expression analysis, airway epithelial, type-I, -II and -III Interferons, epithelial integrity, Biotechnology, TP248.13-248.65 |
| More Details: |
Biosensors such as toll-like receptors (TLR) induce the expression of interferons (IFNs) after viral infection that are critical to the first step in cell-intrinsic host defense mechanisms. Their differential influence on epithelial integrity genes, however, remains elusive. A genome-wide gene expression biosensor chip for gene expression sensing was used to examine the effects of type-I, -II, and -III IFN stimulation on the epithelial expression profiles of primary organotypic 3D air-liquid interface airway cultures. All types of IFNs induced similar interferon-stimulated genes (ISGs): OAS1, OAS2, and IFIT2. However, they differentially induced transcription factors, epithelial modulators, and pro-inflammatory genes. Type-I IFN-induced genes were associated with cell–cell adhesion and tight junctions, while type-III IFNs promoted genes important for transepithelial transport. In contrast, type-II IFN stimulated proliferation-triggering genes associated and enhanced pro-inflammatory mediator secretion. In conclusion, with our microarray system, we provide evidence that the three IFN types exceed their antiviral ISG-response by inducing distinct remodeling processes, thereby likely strengthening the epithelial airway barrier by enhancing cross-cell-integrity (I), transepithelial transport (III) and finally reconstruction through proliferation (II). |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2079-6374 |
| Relation: |
https://www.mdpi.com/2079-6374/12/11/929; https://doaj.org/toc/2079-6374 |
| DOI: |
10.3390/bios12110929 |
| Access URL: |
https://doaj.org/article/347b9773ae1e4dd7b3c47cbc93dafea7 |
| Accession Number: |
edsdoj.347b9773ae1e4dd7b3c47cbc93dafea7 |
| Database: |
Directory of Open Access Journals |
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