Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)

Bibliographic Details
Title:	Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN)
Authors:	Xiaoyu Qi, Qiaoping Mo, Jing Li, Zhibo Zi, Mengyun Xu, Suju Yue, Hongbo Zhao, Haisheng Zhu, Guoping Wang
Source:	Plant Methods, Vol 19, Iss 1, Pp 1-10 (2023)
Publisher Information:	BMC, 2023.
Publication Year:	2023
Collection:	LCC:Plant culture LCC:Biology (General)
Subject Terms:	Luffa, VIGS, Cucumber green mottle mosaic virus (CGMMV), PDS, TEN, Plant culture, SB1-1110, Biology (General), QH301-705.5
More Details:	Abstract Background Virus-induced gene silencing (VIGS) is a reverse genetics technology that can efficiently and rapidly identify plant gene functions. Although a variety of VIGS vectors have been successfully used in plants, only a few reports on VIGS technology in Luffa exist. Results In the present study, a new cucumber green mottle mosaic virus (CGMMV)-based VIGS vector, pV190, was applied to establish the CGMMV-VIGS to investigate the feasibility of the silencing system for Luffa. Phytoene desaturase (PDS) gene was initially selected as a VIGS marker gene to construct a recombinant vector. Plants infected with Agrobacterium harboring pV190-PDS successfully induced effective silencing in Luffa, and an effective gene silencing phenotype with obvious photobleaching was observed. To further validate the efficiency, we selected TEN for gene-silencing, which encodes a CYC/TB1-like transcription factor and is involved in tendril development. Luffa plants inoculated with the pV190-TEN exhibited shorter tendril length and nodal positions where tendrils appear are higher compared to those of non-inoculated plants. RT-qPCR showed that the expression levels of PDS and TEN were significantly reduced in the CGMMV-VIGS plants. Moreover, we evaluated the CGMMV-VIGS efficiency in three cucurbits, including cucumber, ridge gourd, and bottle gourd. Conclusion We successfully established a CGMMV-based VIGS system on ridge gourd and used marker genes to identify the feasibility of the silencing system in Luffa leaves and stems.
Document Type:	article
File Description:	electronic resource
Language:	English
ISSN:	1746-4811
Relation:	https://doaj.org/toc/1746-4811
DOI:	10.1186/s13007-023-01064-4
Access URL:	https://doaj.org/article/3430ccc7747a44cc8838387588014a94
Accession Number:	edsdoj.3430ccc7747a44cc8838387588014a94
Database:	Directory of Open Access Journals
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More Details
ISSN:	17464811
DOI:	10.1186/s13007-023-01064-4
Published in:	Plant Methods
Language:	English