Academic Journal
A longitudinal epidemiology study of fluoroquinolone-nonsusceptible Klebsiella pneumoniae reveals an increasing prevalence of qnrB and qnrS in Taiwan
| Title: | A longitudinal epidemiology study of fluoroquinolone-nonsusceptible Klebsiella pneumoniae reveals an increasing prevalence of qnrB and qnrS in Taiwan |
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| Authors: | Pei-Yun Kuo, Wei-Hung Lin, Shu-Fei Tang, Ya-Yu Cheng, Carl Jay Ballena Bregente, Tran Thi Thuy Duong, Ming-Cheng Wang, Ching-Hao Teng, Yi-Hsien Hsieh, Pei-Fang Tsai, Ying-Chi Li, Cheng-Yen Kao |
| Source: | Journal of Infection and Public Health, Vol 17, Iss 3, Pp 457-463 (2024) |
| Publisher Information: | Elsevier, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Infectious and parasitic diseases LCC:Public aspects of medicine |
| Subject Terms: | Conjugation, Fluoroquinolone-nonsusceptible, Klebsiella pneumoniae, Plasmid-mediated quinolone resistance, qnr, Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270 |
| More Details: | Background: Our objective was to investigate the prevalence of plasmid-mediated quinolone resistance (PMQR) genes in fluoroquinolone-nonsusceptible Klebsiella pneumoniae (FQNSKP) in Taiwan, 1999–2022. Methods: A total of 938 FQNSKP isolates were identified from 1966 isolates. The presence of PMQR and virulence genes, antimicrobial susceptibility, capsular types, and PMQR-plasmid transferability were determined. Results: An increasing number of PMQR-containing FQNSKP isolates were observed over the study period. Our results showed that 69.0% (647 isolates) of FQNSKP isolates contained at least one PMQR gene, and 40.6%, 37.0%, and 33.9% of FQNSKP carried aac(6′)-Ib-cr, qnrB, and qnrS, respectively. None of FQNSKP carried qepA and qnrC. The most common combination of PMQR genes was aac(6′)-Ib-cr and qnrB (12.3%). The presence of PMQR genes is strongly related to resistance to aminoglycoside, cephalosporin, tetracycline, and sulfamethoxazole/trimethoprim in FQNSKP. The capsular serotype K64 is the most common serotype we tested in both the non-PMQR and PMQR FQNSKP isolates, while K20 showed a higher prevalence in PMQR isolates. The magA and peg-344 genes showed a significantly higher prevalence rate in non-PMQR isolates than in PMQR isolates. Eleven isolates that carried the PMQR and carbapenemase genes were identified; however, three successful transconjugants showed that the PMQR and carbapenemase genes were not located on the same plasmid. Conclusions: Our results indicated an increasing prevalence of PMQR genes, especially qnrB and qnrS, in FQNSKP in Taiwan. Moreover, the distribution of PMQR genes was associated with capsular serotypes and antimicrobial resistance gene and virulence gene distribution in FQNSKP. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1876-0341 78528143 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1876034124000054; https://doaj.org/toc/1876-0341 |
| DOI: | 10.1016/j.jiph.2024.01.005 |
| Access URL: | https://doaj.org/article/33f830e8e3924d168f78528143af5a69 |
| Accession Number: | edsdoj.33f830e8e3924d168f78528143af5a69 |
| Database: | Directory of Open Access Journals |
| ISSN: | 18760341 78528143 |
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| DOI: | 10.1016/j.jiph.2024.01.005 |
| Published in: | Journal of Infection and Public Health |
| Language: | English |