Academic Journal
High-intensity interval training reduces the induction of neutrophil extracellular traps in older men using live-neutrophil imaging as biosensor
| Title: | High-intensity interval training reduces the induction of neutrophil extracellular traps in older men using live-neutrophil imaging as biosensor |
|---|---|
| Authors: | Nicolás Vidal-Seguel, Camilo Cabrera, Luciano Ferrada, Macarena Artigas-Arias, Andrea Alegría-Molina, Sergio Sanhueza, Alejandra Flores, Nolberto Huard, Jorge Sapunar, Luis A. Salazar, Reuben McGregor, Estefanía Nova-Lamperti, Gabriel Nasri Marzuca-Nassr |
| Source: | Experimental Gerontology, Vol 181, Iss , Pp 112280- (2023) |
| Publisher Information: | Elsevier, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Medicine LCC:Biology (General) |
| Subject Terms: | NETs, Neutrophil extracellular trap, High-intensity interval training, Exercise, Elderly, Medicine, Biology (General), QH301-705.5 |
| More Details: | Neutrophil extracellular trap formation (NETosis) is a mechanism used by neutrophils to capture pathogens with their own DNA. However, the exacerbation of this immune response is related to serious inflammatory diseases. Aging is known to lead to an excessive increase in NETosis associated with various diseases. Under this scenario, the search for strategies that regulate the release of NETosis in older people becomes relevant. High-intensity interval training (HIIT) involves repeated bouts of relatively intense exercise with alternating short recovery periods. This training has shown beneficial effects on health parameters during aging and disease. However, little is known about the potential role of HIIT in the regulation of NETosis in healthy older people. The aim of this study was to evaluate the induction of NETosis by serum from healthy young and older men, before and after 12 weeks of HIIT using healthy neutrophils as a biosensor. HIIT was performed 3 times per week for 12 weeks in young (YOUNG; 21 ± 1 years, BMI 26.01 ± 2.64 kg⋅m−2, n = 10) and older men (OLDER; 66 ± 5 years, BMI 27.43 ± 3.11 kg⋅m−2, n = 10). Serum samples were taken before and after the HIIT program and NETosis was measured with live cell imaging in donated neutrophils cultured with serum from the participants for 30 h. Our results showed that serum from older men at baseline induced greater baseline NETosis than younger men (p |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1873-6815 49514768 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0531556523002012; https://doaj.org/toc/1873-6815 |
| DOI: | 10.1016/j.exger.2023.112280 |
| Access URL: | https://doaj.org/article/d33d86cf49514768b1d729a4e2e83b1a |
| Accession Number: | edsdoj.33d86cf49514768b1d729a4e2e83b1a |
| Database: | Directory of Open Access Journals |
| ISSN: | 18736815 49514768 |
|---|---|
| DOI: | 10.1016/j.exger.2023.112280 |
| Published in: | Experimental Gerontology |
| Language: | English |