Academic Journal
miR-122/PPARβ axis is involved in hypoxic exercise and modulates fatty acid metabolism in skeletal muscle of obese rats
| Title: | miR-122/PPARβ axis is involved in hypoxic exercise and modulates fatty acid metabolism in skeletal muscle of obese rats |
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| Authors: | Xuebing Wang, Lianshi Feng, Yingli Lu, Haibo Zhang |
| Source: | Heliyon, Vol 10, Iss 4, Pp e26572- (2024) |
| Publisher Information: | Elsevier, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Science (General) LCC:Social sciences (General) |
| Subject Terms: | miR-122, PPARβ, Hypoxic exercise, Fatty acid metabolism, Skeletal muscle, Science (General), Q1-390, Social sciences (General), H1-99 |
| More Details: | Hypoxic exercise is an effective intervention for obesity, because it promotes weight loss by regulating fatty acid (FA) metabolism. The regulation of peroxisome proliferator-activated receptor β (PPARβ) by miR-122 may be involved in this process, but the detailed mechanisms are unknown. In order to address this issue, we probed how miR-122 affected the expression of factors associated with FA metabolism in skeletal muscle of obese rats undergoing hypoxic training. By injecting adeno-associated virus 9 containing miR-122 overexpression vector or miR-122 inhibitor into skeletal muscles of rats with a 4-week hypoxic exercise regimen, the miR-122 expression level can be regulated. Body composition and blood lipid levels were analyzed, and PPARβ, carnitine palmitoyltransferase 1b (CPT1b), acetylCoA carboxylase 2 (ACC2), and FA synthase (FAS) mRNA and protein levels were evaluated using quantitative reverse transcription quantitative PCR(RT-qPCR) and Western blot analysis. We found that miR-122 overexpression increased low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG) levels and decreased PPARβ, ACC2, and FAS expression. Conversely, miR-122 inhibition decreased TG level, increased high-density lipoprotein cholesterol (HDL-C) level, and upregulated PPARβ, ACC2, FAS, and CPT1b. These data indicated that the negative regulation of PPARβ by miR-122 promotes FA metabolism by altering the levels of the factors related to FA metabolism in skeletal muscle of obese rat under hypoxic training, thus providing molecular-level insight into the beneficial effects of this intervention. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2405-8440 88934470 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844024026033; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2024.e26572 |
| Access URL: | https://doaj.org/article/dc3257c88934470b964704fced041eac |
| Accession Number: | edsdoj.3257c88934470b964704fced041eac |
| Database: | Directory of Open Access Journals |
| ISSN: | 24058440 88934470 |
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| DOI: | 10.1016/j.heliyon.2024.e26572 |
| Published in: | Heliyon |
| Language: | English |