Bibliographic Details
| Title: |
Mechanical stretch leads to increased caveolin-1 content and mineralization potential in extracellular vesicles from vascular smooth muscle cells |
| Authors: |
Mohammad Shaver, Kassandra Gomez, Katherine Kaiser, Joshua D. Hutcheson |
| Source: |
BMC Molecular and Cell Biology, Vol 25, Iss 1, Pp 1-12 (2024) |
| Publisher Information: |
BMC, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Cytology |
| Subject Terms: |
Mechanical stretch, Extracellular vesicles, Caveolin-1, Calcification, Vascular smooth muscle cells, Cytology, QH573-671 |
| More Details: |
Abstract Background Hypertension-induced mechanical stress on vascular smooth muscle cells (VSMCs) is a known risk factor for vascular remodeling, including vascular calcification. Caveolin-1 (Cav-1), an integral structural component of plasma membrane invaginations, is a mechanosensitive protein that is required for the formation of calcifying extracellular vesicles (EVs). However, the role of mechanics in Cav-1-induced EV formation from VSMCs has not been reported. Results Exposure of VSMCs to 10% mechanical stretch (0.5 Hz) for 72 h resulted in Cav-1 translocation into non-caveolar regions of the plasma membrane and subsequent redistribution of Cav-1 from the VSMCs into EVs. Inhibition of Rho-A kinase (ROCK) in mechanically-stimulated VSMCs exacerbated the liberation of Cav-1 positive EVs from the cells, suggesting a potential involvement of actin stress fibers in this process. The mineralization potential of EVs was measured by incubating the EVs in a high phosphate solution and measuring light scattered by the minerals at 340 nm. EVs released from stretched VSMCs showed higher mineralization potential than the EVs released from non-stretched VSMCs. Culturing VSMCs in pro-calcific media and exposure to mechanical stretch increased tissue non-specific alkaline phosphatase (ALP), an important enzyme in vascular calcification, activity in EVs released from the cells, with cyclic stretch further elevating EV ALP activity compared to non-stretched cells. Conclusion Our data demonstrate that mechanical stretch alters Cav-1 trafficking and EV release, and the released EVs have elevated mineralization potential. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2661-8850 |
| Relation: |
https://doaj.org/toc/2661-8850 |
| DOI: |
10.1186/s12860-024-00504-w |
| Access URL: |
https://doaj.org/article/d3049e26385641fba3a71b51b8fd9349 |
| Accession Number: |
edsdoj.3049e26385641fba3a71b51b8fd9349 |
| Database: |
Directory of Open Access Journals |
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