Bibliographic Details
| Title: |
RNA binding profile analysis of N-acetyltransferase 10 in K562 cells |
| Authors: |
LI Gaoyuan, WANG Yanran, WANG Fang, YU Jia |
| Source: |
Jichu yixue yu linchuang, Vol 43, Iss 5, Pp 739-744 (2023) |
| Publisher Information: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College., 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Medicine |
| Subject Terms: |
n-acetyltransferase 10, k562 cells, enhanced uv crosslinking, immunoprecipitation, and high-throughput sequencing(eclip-seq), Medicine |
| More Details: |
Objective To delineate RNA binding profile of NAT10(N-acetyltransferase 10) in human chronic myelogenous leukemia cell line K562. Methods Enhanced UV crosslinking and immunoprecipitation (eCLIP) were used to capture the transcript collection bound by NAT10 in K562 cells. Data were obtained through high throughput sequencing and then analyzed with bioinformatics. The type and regions of NAT10 binding genes were identified via peak-calling and annotation. The function of the binding RNA was further explored by gene function enrichment analysis. Results NAT10 binding transcripts which were enriched in 3′UTR region, were mainly protein coding genes. Further analysis showed that they were functionally associated with DNA damage and repair. Conclusions NAT10 may regulate gene expression by binding to the 3′UTR region of mRNA associated with DNA damage repair. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
Chinese |
| ISSN: |
1001-6325 |
| Relation: |
http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-5-739.pdf; https://doaj.org/toc/1001-6325 |
| DOI: |
10.16352/j.issn.1001-6325.2023.05.0739 |
| Access URL: |
https://doaj.org/article/2fec463f930441cab0ae63674ea365ca |
| Accession Number: |
edsdoj.2fec463f930441cab0ae63674ea365ca |
| Database: |
Directory of Open Access Journals |
