Bibliographic Details
| Title: |
High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples |
| Authors: |
Emily M. Thrash, Katja Kleinsteuber, Emma S. Hathaway, Matthew Nazzaro, Eric Haas, F. Stephen Hodi, Mariano Severgnini |
| Source: |
STAR Protocols, Vol 1, Iss 2, Pp 100055- (2020) |
| Publisher Information: |
Elsevier, 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Science (General) |
| Subject Terms: |
Science (General), Q1-390 |
| More Details: |
Summary: As mass cytometry (MC) is implemented in clinical settings, the need for robust, validated protocols that reduce batch effects between samples becomes increasingly important. Here, we present a streamlined MC workflow for high-throughput staining that generates reproducible data for up to 80 samples in a single experiment by combining reference sample spike-in and palladium-based mass-tag cell barcoding. Although labor intensive, this workflow decreases experimental variables and thus reduces technical error and mitigates batch effects. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2666-1667 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666166720300423; https://doaj.org/toc/2666-1667 |
| DOI: |
10.1016/j.xpro.2020.100055 |
| Access URL: |
https://doaj.org/article/2f78f90cca67436ca5cc2859d58bed32 |
| Accession Number: |
edsdoj.2f78f90cca67436ca5cc2859d58bed32 |
| Database: |
Directory of Open Access Journals |
