Cinobufagin disrupts the stability of lipid rafts by inhibiting the expression of caveolin-1 to promote non-small cell lung cancer cell apoptosis
| Title: | Cinobufagin disrupts the stability of lipid rafts by inhibiting the expression of caveolin-1 to promote non-small cell lung cancer cell apoptosis |
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| Authors: | Zhongqing Xu, Jinwei Li, Shuyu Fang, Mingzhu Lian, Changxiao Zhang, Jiahuan Lu, Kai Sheng |
| Source: | Archives of Medical Science, Vol 20, Iss 3, Pp 887-908 (2024) |
| Publisher Information: | Termedia Publishing House, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Medicine |
| Subject Terms: | non-small cell lung cancer, cinobufagin, lipid rafts, caveolin-1, Medicine |
| More Details: | Introduction The study was designed to explore how cinobufagin (CB) regulates the development of non-small cell lung cancer (NSCLC) cells through lipid rafts. Material and methods The effects of CB at gradient concentrations (0, 0.5, 1 and 2 µM) on NSCLC cell viability, apoptosis, reactive oxygen species (ROS) level, phosphorylation of Akt, and apoptosis- and lipid raft-related protein expression were assessed by MTT assay, flow cytometry and Western blot. Cholesterol and sphingomyelin were labeled with BODIPY to evaluate the effect of CB (2 µM) on them. Sucrose density gradient centrifugation was used to extract lipid rafts. The effect of CB on the expression and distribution of caveolin-1 was determined by immunofluorescence, quantitative reverse transcription polymerase chain reaction and Western blot. After overexpression of caveolin-1, the above experiments were performed again to observe whether the regulatory effect of CB was reversed. Results CB inhibited NSCLC cell viability while promoting apoptosis and ROS level. CB redistributed the lipid content on the membrane surface and reduced the content of caveolin-1 in the cell membrane. In addition, CB repressed the activation of AKT. However, caveolin-1 overexpression reversed the effects of CB on apoptosis, AKT activation and lipid raft. Conclusions CB regulates the activity of Akt in lipid rafts by inhibiting caveolin-1 expression to promote NSCLC cell apoptosis. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1734-1922 1896-9151 |
| Relation: | https://www.archivesofmedicalscience.com/Cinobufagin-disrupts-the-stability-of-lipid-rafts-by-inhibiting-the-expression-of,174578,0,2.html; https://doaj.org/toc/1734-1922; https://doaj.org/toc/1896-9151 |
| DOI: | 10.5114/aoms/174578 |
| Access URL: | https://doaj.org/article/2df9d574647646af8ad37b89f852d840 |
| Accession Number: | edsdoj.2df9d574647646af8ad37b89f852d840 |
| Database: | Directory of Open Access Journals |
| ISSN: | 17341922 18969151 |
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| DOI: | 10.5114/aoms/174578 |
| Published in: | Archives of Medical Science |
| Language: | English |