Bibliographic Details
| Title: |
Experimental infectious challenge in pigs leads to elevated fecal calprotectin levels following colitis, but not enteritis |
| Authors: |
Jéssica A. Barbosa, Lucas A. Rodrigues, Daniel A. Columbus, Juan C. P. Aguirre, John C. S. Harding, Vinícius S. Cantarelli, Matheus de O. Costa |
| Source: |
Porcine Health Management, Vol 7, Iss 1, Pp 1-9 (2021) |
| Publisher Information: |
BMC, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Animal culture
LCC:Veterinary medicine |
| Subject Terms: |
Biological markers, Intestinal inflammation, Enteric disease, Swine, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| More Details: |
Abstract Background Fecal calprotectin is largely applied as a non-invasive intestinal inflammation biomarker in human medicine. Previous studies in pigs investigated the levels of fecal calprotectin in healthy animals only. Thus, there is a knowledge gap regarding its application during infectious diarrhea. This study investigated the usefulness of fecal calprotectin as a biomarker of intestinal inflammation in Brachyspira hyodysenteriae and Salmonella Typhimurium infected pigs. Results Fecal samples from pigs with colitis (n = 18) were collected from animals experimentally inoculated with B. hyodysenteriae (n = 8) or from sham-inoculated controls (n = 3). Fecal samples from pigs with enteritis (n = 14) were collected from animals inoculated with Salmonella enterica serovar Typhimurium (n = 8) or from sham-inoculated controls (n = 4). For both groups, fecal samples were scored as: 0 = normal; 1 = soft, wet cement; 2 = watery feces; 3 = mucoid diarrhea; and 4 = bloody diarrhea. Fecal calprotectin levels were assayed using a sandwich ELISA, a turbidimetric immunoassay and a point-of-care dipstick test. Fecal calprotectin levels were greater in colitis samples scoring 4 versus ≤ 4 using ELISA, and in feces scoring 3 and 4 versus ≤ 1 using immunoturbidimetry (P |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2055-5660 |
| Relation: |
https://doaj.org/toc/2055-5660 |
| DOI: |
10.1186/s40813-021-00228-9 |
| Access URL: |
https://doaj.org/article/2de59bd4f24b4bc1ac752c23cb9a6c32 |
| Accession Number: |
edsdoj.2de59bd4f24b4bc1ac752c23cb9a6c32 |
| Database: |
Directory of Open Access Journals |
