Bibliographic Details
| Title: |
Characterization of blood-derived exosomal hTERT mRNA as a biomarker for colon cancer and Lynch syndrome |
| Authors: |
Ido Laish, Zohar Levi, Hussein Mahajna, Ahmad Albshesh, Nir Horesh, Efraim Katz, Dan Feldman, Nadav Shinar, Orit Picard, Miri Yavzori, Ella Fudim, Pia Raanani, Tamar Berger, Hadar Goldvaser, Einat Beery, Orit Uziel |
| Source: |
Frontiers in Oncology, Vol 12 (2022) |
| Publisher Information: |
Frontiers Media S.A., 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| Subject Terms: |
telomerases, exosomes, colon cancer, Lynch syndrome, genetic syndromes, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| More Details: |
BackgroundHuman telomerase reverse transcriptase (hTERT)- mRNA was shown to be elevated in exosomes derived from the sera of a variety of hematological and solid cancer patients. We aimed to evaluate its role as a diagnostic marker in patients with newly diagnosed colon cancer and in hereditary syndromes with predisposition to colon cancer.MethodshTERT -mRNA levels were determined in serum-derived exosomes from 88 patients with colon cancer, 71 Lynch-syndrome carriers with unknown active malignancies and 50 healthy controls. Data, including demographics, background diseases, clinical data regarding tumor characteristics and genetic data, were retrieved data from medical files.ResultsPatients with colon cancer had both higher exosomal hTERT mRNA levels and a higher proportion of patients with positive exosomal hTERT mRNA than controls (29.5% vs. 4%, respectively, P values < 0.001). Within the cancer group, patients with a metastatic disease had higher levels of telomerase mRNA than non-metastatic disease patients, and these levels correlated with CEA levels. Likewise, Lynch syndrome carriers had a higher proportion of positive exosomal hTERT mRNA than controls (21.1% vs. 4%, respectively, P value 0.008) but only a trend towards higher exosomal hTERT mRNA levels. Higher telomerase mRNA levels were not correlated with the mutated gene.ConclusionsExosomal serum hTERT –mRNA levels are associated with metastatic colon cancer and were also demonstrated in a subset of Lynch syndrome carriers. Its significance as a biomarker for developing malignancy should be elucidated. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2234-943X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fonc.2022.962473/full; https://doaj.org/toc/2234-943X |
| DOI: |
10.3389/fonc.2022.962473 |
| Access URL: |
https://doaj.org/article/2d86f3df872f47318903ba82f9c23c8f |
| Accession Number: |
edsdoj.2d86f3df872f47318903ba82f9c23c8f |
| Database: |
Directory of Open Access Journals |
