Bibliographic Details
| Title: |
A Molecular-Level Account of the Antigenic Hantaviral Surface |
| Authors: |
Sai Li, Ilona Rissanen, Antra Zeltina, Jussi Hepojoki, Jayna Raghwani, Karl Harlos, Oliver G. Pybus, Juha T. Huiskonen, Thomas A. Bowden |
| Source: |
Cell Reports, Vol 15, Iss 5, Pp 959-967 (2016) |
| Publisher Information: |
Elsevier, 2016. |
| Publication Year: |
2016 |
| Collection: |
LCC:Biology (General) |
| Subject Terms: |
Biology (General), QH301-705.5 |
| More Details: |
Hantaviruses, a geographically diverse group of zoonotic pathogens, initiate cell infection through the concerted action of Gn and Gc viral surface glycoproteins. Here, we describe the high-resolution crystal structure of the antigenic ectodomain of Gn from Puumala hantavirus (PUUV), a causative agent of hemorrhagic fever with renal syndrome. Fitting of PUUV Gn into an electron cryomicroscopy reconstruction of intact Gn-Gc spike complexes from the closely related but non-pathogenic Tula hantavirus localized Gn tetramers to the membrane-distal surface of the virion. The accuracy of the fitting was corroborated by epitope mapping and genetic analysis of available PUUV sequences. Interestingly, Gn exhibits greater non-synonymous sequence diversity than the less accessible Gc, supporting a role of the host humoral immune response in exerting selective pressure on the virus surface. The fold of PUUV Gn is likely to be widely conserved across hantaviruses. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2211-1247 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2211124716303849; https://doaj.org/toc/2211-1247 |
| DOI: |
10.1016/j.celrep.2016.03.082 |
| Access URL: |
https://doaj.org/article/2d7f19f22de2439fada439d2843905af |
| Accession Number: |
edsdoj.2d7f19f22de2439fada439d2843905af |
| Database: |
Directory of Open Access Journals |
