An integrated workflow for crosslinking mass spectrometry

Bibliographic Details
Title:	An integrated workflow for crosslinking mass spectrometry
Authors:	Marta L Mendes, Lutz Fischer, Zhuo A Chen, Marta Barbon, Francis J O'Reilly, Sven H Giese, Michael Bohlke‐Schneider, Adam Belsom, Therese Dau, Colin W Combe, Martin Graham, Markus R Eisele, Wolfgang Baumeister, Christian Speck, Juri Rappsilber
Source:	Molecular Systems Biology, Vol 15, Iss 9, Pp 1-13 (2019)
Publisher Information:	Springer Nature, 2019.
Publication Year:	2019
Collection:	LCC:Biology (General) LCC:Medicine (General)
Subject Terms:	crosslinking mass spectrometry, protein–protein interactions, proteomics, software, structural biology, Biology (General), QH301-705.5, Medicine (General), R5-920
More Details:	Abstract We present a concise workflow to enhance the mass spectrometric detection of crosslinked peptides by introducing sequential digestion and the crosslink identification software xiSEARCH. Sequential digestion enhances peptide detection by selective shortening of long tryptic peptides. We demonstrate our simple 12‐fraction protocol for crosslinked multi‐protein complexes and cell lysates, quantitative analysis, and high‐density crosslinking, without requiring specific crosslinker features. This overall approach reveals dynamic protein–protein interaction sites, which are accessible, have fundamental functional relevance and are therefore ideally suited for the development of small molecule inhibitors.
Document Type:	article
File Description:	electronic resource
Language:	English
ISSN:	1744-4292
Relation:	https://doaj.org/toc/1744-4292
DOI:	10.15252/msb.20198994
Access URL:	https://doaj.org/article/dc2c1aa4ba3649908c7432cf24169792
Accession Number:	edsdoj.2c1aa4ba3649908c7432cf24169792
Database:	Directory of Open Access Journals
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More Details
ISSN:	17444292
DOI:	10.15252/msb.20198994
Published in:	Molecular Systems Biology
Language:	English