Bibliographic Details
| Title: |
Biological Characteristics of a Novel Bibenzyl Synthase (DoBS1) Gene from Dendrobium officinale Catalyzing Dihydroresveratrol Synthesis |
| Authors: |
Shao-Guo Zhou, Ke Zhong, Feng-Xia Yan, Fan Tian, Chang-Sha Luo, Hang-Cheng Yu, Zai-Qi Luo, Xi-Min Zhang |
| Source: |
Molecules, Vol 29, Iss 22, p 5320 (2024) |
| Publisher Information: |
MDPI AG, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Organic chemistry |
| Subject Terms: |
bibenzyl synthase, Dendrobium officinale, dihydroresveratrol, enzyme activity, in vitro biosynthesis, Organic chemistry, QD241-441 |
| More Details: |
Bibenzyl compounds are one of the most important bioactive components of natural medicine. However, Dendrobium officinale as a traditional herbal medicine is rich in bibenzyl compounds and performs functions such as acting as an antioxidant, inhibiting cancer cell growth, and assisting in neuro-protection. The biosynthesis of bibenzyl products is regulated by bibenzyl synthase (BBS). In this study, we have cloned the cDNA gene of the bibenzyl synthase (DoBS1) from D. officinale using PCR with degenerate primers, and we have identified a novel type III polyketide synthase (PKS) gene by phylogenetic analyses. In a series of perfect experiments, DoBS1 was expressed in Escherichia coli, purified and some catalytic properties of the recombinant protein were investigated. The molecular weight of the recombinant protein was verified to be approximately 42.7 kDa. An enzyme activity analysis indicated that the recombinant DoBS1-HisTag protein was capable of using 4-coumaryol-CoA and 3 malonyl-CoA as substrates for dihydroresveratrol (DHR) in vitro. The Vmax and Km of the recombinant protein for DHR were 3.57 ± 0.23 nmol·min−1·mg−1 and 0.30 ± 0.08 mmol, respectively. The present study provides further insights into the catalytic mechanism of the active site in the biosynthetic pathway for the catalytic production of dihydroresveratrol by bibenzylase in D. officinale. The results can be used to optimize a novel biosynthetic pathway for the industrial synthesis of DHR. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1420-3049 |
| Relation: |
https://www.mdpi.com/1420-3049/29/22/5320; https://doaj.org/toc/1420-3049 |
| DOI: |
10.3390/molecules29225320 |
| Access URL: |
https://doaj.org/article/2aa1701f3abf452482e2363d445c36f0 |
| Accession Number: |
edsdoj.2aa1701f3abf452482e2363d445c36f0 |
| Database: |
Directory of Open Access Journals |
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