Bibliographic Details
| Title: |
Generation of a USP9Y knockout human embryonic stem cell line with CRISPR-Cas9 technology |
| Authors: |
Sai Wei, Yuting Zhen, Chao Sun, Yanlin Ma, Qi Li, Luan Wen |
| Source: |
Stem Cell Research, Vol 82, Iss , Pp 103646- (2025) |
| Publisher Information: |
Elsevier, 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Biology (General) |
| Subject Terms: |
Biology (General), QH301-705.5 |
| More Details: |
Human embryonic stem cell (hESC) lines are vital tools for studying gene function, disease modeling, and therapy. We generated a USP9Y knockout hESC line using CRISPR-Cas9 in the male-derived H1 line. Targeted deletion of the USP9Y gene was confirmed via PCR and sequencing. The modified line retained pluripotency markers, exhibited a normal karyotype, and differentiated into all three germ layers. This model provides a valuable platform for studying USP9Y’s role in human development and male infertility, offering insights into related disorders and therapeutic potential. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1873-5061 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1873506124003441; https://doaj.org/toc/1873-5061 |
| DOI: |
10.1016/j.scr.2024.103646 |
| Access URL: |
https://doaj.org/article/2a78d0f8ee9d4f389705c786d64d5c5c |
| Accession Number: |
edsdoj.2a78d0f8ee9d4f389705c786d64d5c5c |
| Database: |
Directory of Open Access Journals |
