Bibliographic Details
| Title: |
Proteomic analysis of 2-chloroethanol extracts of rice (Oryza sativa L.) seeds |
| Authors: |
Songlin Ruan, Wenfei Xiao, Jieren Qiu, Weimin Hu, Wu Ying, Huizhe Chen, Jianxin Tong, Huasheng Ma |
| Source: |
Food Chemistry: Molecular Sciences, Vol 1, Iss , Pp 100002- (2020) |
| Publisher Information: |
Elsevier, 2020. |
| Publication Year: |
2020 |
| Collection: |
LCC:Nutrition. Foods and food supply |
| Subject Terms: |
Rice seed, Ethanol-soluble protein, Proteomic analysis, Seed storage protein, Protease inhibitors, Nutrition. Foods and food supply, TX341-641 |
| More Details: |
Ethanol-soluble proteins, including prolamins, are one of the most important seed proteins in rice (Oryza sativa L.). However, little is known about the proteomic profile of ethanol-soluble protein fraction extracted from rice grain. In this work, the differential profile of ethanol-soluble proteins extracted by 2-chloroethanol and ethanol has been documented. Proteome analysis utilizing LC-MS/MS identified a total of 64 unique proteins in the 2-chloroethanol extract of rice seeds. The majority of these proteins had low molecular weight ranging from 10 to 25 kD and isoelectric point (pI) in mid-acidic (pH 5–pH 7) and mid-basic (pH 7–pH 9) ranges. Database searches combined with transmembrane domain (TMD) analysis revealed that >70% of identified proteins were hydrophobic, i.e., had at least one TMD. Gene ontology classification and enrichment analysis showed that the identified proteins were involved in13 types of biological processes, 5 types of cell components, and 17 types of molecular functions. These results were significant based on the hyper p-value of |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2666-5662 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666566220300022; https://doaj.org/toc/2666-5662 |
| DOI: |
10.1016/j.fochms.2020.100002 |
| Access URL: |
https://doaj.org/article/d2a220b59c3b4ea8be1aa4b83c75586d |
| Accession Number: |
edsdoj.2a220b59c3b4ea8be1aa4b83c75586d |
| Database: |
Directory of Open Access Journals |
