Bibliographic Details
| Title: |
Expression and localization of absent in melanoma 2 in the injured spinal cord |
| Authors: |
Sai-Nan Wang, Xue-Yan Guo, Jie Tang, Shu-Qin Ding, Lin Shen, Rui Wang, Shan-Feng Ma, Jian-Guo Hu, He-Zuo Lü |
| Source: |
Neural Regeneration Research, Vol 14, Iss 3, Pp 542-552 (2019) |
| Publisher Information: |
Wolters Kluwer Medknow Publications, 2019. |
| Publication Year: |
2019 |
| Collection: |
LCC:Neurology. Diseases of the nervous system |
| Subject Terms: |
nerve regeneration, spinal cord injury, absent in melanoma 2, spatio-temporal expression, neurons, astrocytes, oligodendrocytes, infiltrated leukocytes, activated microglia, western blot assay, immunohistochemistry, neural regeneration, Neurology. Diseases of the nervous system, RC346-429 |
| More Details: |
In traumatic brain injury, absent in melanoma 2 (AIM2) has been demonstrated to be involved in pyroptotic neuronal cell death. Although the pathophysiological mechanism of spinal cord injury is similar to that of brain injury, the expression and cellular localization of AIM2 after spinal cord injury is still not very clear. In the present study, we used a rat model of T9 spinal cord contusive injury, produced using the weight drop method. The rats were randomly divided into 1-hour, 6-hour, 1-day, 3-day and 6-day (post-injury time points) groups. Sham-operated rats only received laminectomy at T9 without contusive injury. Western blot assay revealed that the expression levels of AIM2 were not significantly different among the 1-hour, 6-hour and 1-day groups. The expression levels of AIM2 were markedly higher in the 1-hour, 6-hour and 1-day groups compared with the sham, 3-day and 7-day groups. Double immunofluorescence staining demonstrated that AIM2 was expressed by NeuN+ (neurons), GFAP+ (astrocytes), CNPase+ (oligodendrocytes) and CD11b+ (microglia) cells in the sham-operated spinal cord. In rats with spinal cord injury, AIM2 was also found in CD45+ (leukocytes) and CD68+ (activated microglia/macrophages) cells in the spinal cord at all time points. These findings indicate that AIM2 is mainly expressed in neurons, astrocytes, microglia and oligodendrocytes in the normal spinal cord, and that after spinal cord injury, its expression increases because of the infiltration of leukocytes and the activation of astrocytes and microglia/macrophages. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1673-5374 |
| Relation: |
http://www.nrronline.org/article.asp?issn=1673-5374;year=2019;volume=14;issue=3;spage=542;epage=552;aulast=Wang; https://doaj.org/toc/1673-5374 |
| DOI: |
10.4103/1673-5374.245481 |
| Access URL: |
https://doaj.org/article/29e2d5ba6b924690b4f7017a6a07c61f |
| Accession Number: |
edsdoj.29e2d5ba6b924690b4f7017a6a07c61f |
| Database: |
Directory of Open Access Journals |
