Bibliographic Details
| Title: |
Effect of the genetic mutant G71R in uridine diphosphate-glucuronosyltransferase 1A1 on the conjugation of bilirubin |
| Authors: |
Chen Hong, Zhong Danni, Gao Zongyan, Wu Xiaojing |
| Source: |
Open Life Sciences, Vol 17, Iss 1, Pp 221-229 (2022) |
| Publisher Information: |
De Gruyter, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Biology (General) |
| Subject Terms: |
lentiviral vector, g71r mutant, hyperbilirubinemia, udp-glucuronosyltransferase 1a1, Biology (General), QH301-705.5 |
| More Details: |
We aimed to investigate the effect of the genetic mutant G71R (c. 211G > A) in uridine diphosphate (UDP)-glucuronosyltransferase 1A1 (UGT1A1) on the glucuronidation of unconjugated bilirubin. The UGT1A1 wild-type and mutant G71R gene sequences were inserted into the lentiviral vector GV358 plasmid and then transfected into COS-7 cells. Real-time polymerase chain reaction and western blot analyses were used to determine mRNA and protein expression levels of UGT1A1, respectively. High-performance liquid chromatography was used to quantitate the levels of conjugated bilirubin. The results showed no significant difference in the mRNA and protein expression levels between the UGT1A1 wild-type and G71R homozygous and heterozygous mutants. The level of conjugated bilirubin reached a maximum in wild-type UGT1A1-transfected COS-7 cells. However, relative to the UGT1A1 wild-type, conjugated bilirubin concentrations were 71 and 22% with G71R heterozygous- and G71R homozygous-transfected COS-7 cells, respectively. In conclusion, we successfully established in vitro cell models of the UGT1A1 wild-type and the G71R homozygous and heterozygous mutants using a lentiviral vector. Furthermore, the catalytic activity for unconjugated bilirubin was lower in the mutant G71R than the UGT1A1 wild-type enzyme, and a weaker effect was observed in the homozygote. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2391-5412 |
| Relation: |
https://doaj.org/toc/2391-5412 |
| DOI: |
10.1515/biol-2022-0021 |
| Access URL: |
https://doaj.org/article/2966bbc83cd5465b8c756528edb4e9ae |
| Accession Number: |
edsdoj.2966bbc83cd5465b8c756528edb4e9ae |
| Database: |
Directory of Open Access Journals |
