Bibliographic Details
| Title: |
Cardiac progenitor cell-derived extracellular vesicles promote angiogenesis through both associated- and co-isolated proteins |
| Authors: |
Marieke Theodora Roefs, Julia Bauzá-Martinez, Simonides Immanuel van de Wakker, Jiabin Qin, Willem Theodoor Olijve, Robin Tuinte, Marjolein Rozeboom, Christian Snijders Blok, Emma Alise Mol, Wei Wu, Pieter Vader, Joost Petrus Gerardus Sluijter |
| Source: |
Communications Biology, Vol 6, Iss 1, Pp 1-16 (2023) |
| Publisher Information: |
Nature Portfolio, 2023. |
| Publication Year: |
2023 |
| Collection: |
LCC:Biology (General) |
| Subject Terms: |
Biology (General), QH301-705.5 |
| More Details: |
Abstract Extracellular vesicles (EVs) are cell-derived lipid bilayer-enclosed particles that play a role in intercellular communication. Cardiac progenitor cell (CPC)-derived EVs have been shown to protect the myocardium against ischemia-reperfusion injury via pro-angiogenic effects. However, the mechanisms underlying CPC-EV-induced angiogenesis remain elusive. Here, we discovered that the ability of CPC-EVs to induce in vitro angiogenesis and to stimulate pro-survival pathways was lost upon EV donor cell exposure to calcium ionophore. Proteomic comparison of active and non-active EV preparations together with phosphoproteomic analysis of activated endothelial cells identified the contribution of candidate protein PAPP-A and the IGF-R signaling pathway in EV-mediated cell activation, which was further validated using in vitro angiogenesis assays. Upon further purification using iodixanol gradient ultracentrifugation, EVs partly lost their activity, suggesting a co-stimulatory role of co-isolated proteins in recipient cell activation. Our increased understanding of the mechanisms of CPC-EV-mediated cell activation will pave the way to more efficient EV-based therapeutics. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2399-3642 |
| Relation: |
https://doaj.org/toc/2399-3642 |
| DOI: |
10.1038/s42003-023-05165-7 |
| Access URL: |
https://doaj.org/article/27db586875664b9cb4dbdeb36d2d3c0f |
| Accession Number: |
edsdoj.27db586875664b9cb4dbdeb36d2d3c0f |
| Database: |
Directory of Open Access Journals |
