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Cross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia

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Title: Cross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia
Authors: Samy Kasem, Ibraheem Qasim, Ali Al-Hufofi, Osman Hashim, Ali Alkarar, Ali Abu-Obeida, Albagir Gaafer, Abdelhamid Elfadil, Ahmed Zaki, Ahmed Al-Romaihi, Nasereldeen Babekr, Nadr El-Harby, Raed Hussien, Ali AL-Sahaf, Ali Al-Doweriej, Faisal Bayoumi, Leo L.M. Poon, Daniel K.W. Chu, Malik Peiris, Ranawaka A.P.M. Perera
Source: Journal of Infection and Public Health, Vol 11, Iss 3, Pp 331-338 (2018)
Publisher Information: Elsevier, 2018.
Publication Year: 2018
Collection: LCC:Infectious and parasitic diseases
LCC:Public aspects of medicine
Subject Terms: Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270
More Details: Background: Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the Middle East. The epidemiological profiles of the MERS-CoV infections suggest zoonotic transmission from an animal reservoir to humans. Methods: This study was designed to investigate animal herds associated with Middle East respiratory syndrome (MERS)-infected patients in Saudi Arabia, during the last three years (2014–2016). Nasal swabs and serum samples from 584 dromedary camels, 39 sheep, 51 goats, and 2 cattle were collected. Nasal samples from camels, sheep, goats, and cattle were examined by real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV RNA, and the Anti-MERS ELISA assay was performed to detect camel humeral immune response (IgG) to MERS-CoV S1 antigen infection. The complete genome sequencing of ten MERS-CoV camel isolates and phylogenetic analysis was performed. Results: The data indicated that seventy-five dromedary camels were positive for MERS-CoV RNA; the virus was not detected in sheep, goats, and cattle. MERS-CoV RNA from infected camels was not detected beyond 2 weeks after the first positive result was detected in nasal swabs obtained from infected camels. Anti-MERS ELISA assays showed that 70.9% of camels related to human cases had antibodies to MERS-CoV. The full genome sequences of the ten MERS-CoV camel isolates were identical to their corresponding patients and were grouped together within the larger MERS-CoV sequences cluster for human and camel isolates reported form the Arabian Peninsula. Conclusions: These findings indicate that camels are a significant reservoir for the maintenance of MERS-CoVs, and they are an important source of human infection with MERS. Keywords: MERS, Dromedary camel, Real time-PCR, ELISA, Saudi Arabia
Document Type: article
File Description: electronic resource
Language: English
ISSN: 1876-0341
Relation: http://www.sciencedirect.com/science/article/pii/S1876034117302575; https://doaj.org/toc/1876-0341
DOI: 10.1016/j.jiph.2017.09.022
Access URL: https://doaj.org/article/c2746ecd6f41449887b84939d411e7a5
Accession Number: edsdoj.2746ecd6f41449887b84939d411e7a5
Database: Directory of Open Access Journals
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  Data: Cross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia
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  Data: <searchLink fieldCode="AR" term="%22Samy+Kasem%22">Samy Kasem</searchLink><br /><searchLink fieldCode="AR" term="%22Ibraheem+Qasim%22">Ibraheem Qasim</searchLink><br /><searchLink fieldCode="AR" term="%22Ali+Al-Hufofi%22">Ali Al-Hufofi</searchLink><br /><searchLink fieldCode="AR" term="%22Osman+Hashim%22">Osman Hashim</searchLink><br /><searchLink fieldCode="AR" term="%22Ali+Alkarar%22">Ali Alkarar</searchLink><br /><searchLink fieldCode="AR" term="%22Ali+Abu-Obeida%22">Ali Abu-Obeida</searchLink><br /><searchLink fieldCode="AR" term="%22Albagir+Gaafer%22">Albagir Gaafer</searchLink><br /><searchLink fieldCode="AR" term="%22Abdelhamid+Elfadil%22">Abdelhamid Elfadil</searchLink><br /><searchLink fieldCode="AR" term="%22Ahmed+Zaki%22">Ahmed Zaki</searchLink><br /><searchLink fieldCode="AR" term="%22Ahmed+Al-Romaihi%22">Ahmed Al-Romaihi</searchLink><br /><searchLink fieldCode="AR" term="%22Nasereldeen+Babekr%22">Nasereldeen Babekr</searchLink><br /><searchLink fieldCode="AR" term="%22Nadr+El-Harby%22">Nadr El-Harby</searchLink><br /><searchLink fieldCode="AR" term="%22Raed+Hussien%22">Raed Hussien</searchLink><br /><searchLink fieldCode="AR" term="%22Ali+AL-Sahaf%22">Ali AL-Sahaf</searchLink><br /><searchLink fieldCode="AR" term="%22Ali+Al-Doweriej%22">Ali Al-Doweriej</searchLink><br /><searchLink fieldCode="AR" term="%22Faisal+Bayoumi%22">Faisal Bayoumi</searchLink><br /><searchLink fieldCode="AR" term="%22Leo+L%2EM%2E+Poon%22">Leo L.M. Poon</searchLink><br /><searchLink fieldCode="AR" term="%22Daniel+K%2EW%2E+Chu%22">Daniel K.W. Chu</searchLink><br /><searchLink fieldCode="AR" term="%22Malik+Peiris%22">Malik Peiris</searchLink><br /><searchLink fieldCode="AR" term="%22Ranawaka+A%2EP%2EM%2E+Perera%22">Ranawaka A.P.M. Perera</searchLink>
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  Data: Background: Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the Middle East. The epidemiological profiles of the MERS-CoV infections suggest zoonotic transmission from an animal reservoir to humans. Methods: This study was designed to investigate animal herds associated with Middle East respiratory syndrome (MERS)-infected patients in Saudi Arabia, during the last three years (2014–2016). Nasal swabs and serum samples from 584 dromedary camels, 39 sheep, 51 goats, and 2 cattle were collected. Nasal samples from camels, sheep, goats, and cattle were examined by real-time reverse-transcription PCR (RT-PCR) to detect MERS-CoV RNA, and the Anti-MERS ELISA assay was performed to detect camel humeral immune response (IgG) to MERS-CoV S1 antigen infection. The complete genome sequencing of ten MERS-CoV camel isolates and phylogenetic analysis was performed. Results: The data indicated that seventy-five dromedary camels were positive for MERS-CoV RNA; the virus was not detected in sheep, goats, and cattle. MERS-CoV RNA from infected camels was not detected beyond 2 weeks after the first positive result was detected in nasal swabs obtained from infected camels. Anti-MERS ELISA assays showed that 70.9% of camels related to human cases had antibodies to MERS-CoV. The full genome sequences of the ten MERS-CoV camel isolates were identical to their corresponding patients and were grouped together within the larger MERS-CoV sequences cluster for human and camel isolates reported form the Arabian Peninsula. Conclusions: These findings indicate that camels are a significant reservoir for the maintenance of MERS-CoVs, and they are an important source of human infection with MERS. Keywords: MERS, Dromedary camel, Real time-PCR, ELISA, Saudi Arabia
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