New tissue microarray technology for analyses of gene expression in frozen pathological samples
| Title: | New tissue microarray technology for analyses of gene expression in frozen pathological samples |
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| Authors: | Lei Zhou, Melissa Hodeib, Joseph D. Abad, Leopoldo Mendoza, Anilkumar R. Kore, Zhongting Hu |
| Source: | BioTechniques, Vol 43, Iss 1, Pp 101-105 (2007) |
| Publisher Information: | Taylor & Francis Group, 2007. |
| Publication Year: | 2007 |
| Collection: | LCC:Biology (General) |
| Subject Terms: | Biology (General), QH301-705.5 |
| More Details: | Tissue microarrays (TMAs) are widely used to analyze gene expression in multiple pathological samples on a single slide. Currently, most TMA slides are made by coring paraffinembedded tissues and arraying them into a paraffin block, from which TMA sections are cut. However, paraffin-based TMA technology may not be compatible with frozen clinical tissue samples, which have a higher quality of RNAs and proteins for preparing TMAs than paraffin-embedded tissue samples. In this study, we developed an alternative TMA technology that is applicable to a broader range of frozen tissue samples. Our method takes advantage of a newly developed array recipient block that can be used to array small tissue cores. After arraying tissue cores, the tissue block can be immediately sectioned on a cryostat microtome to make TMA slides. TMAs made using this method have well-defined array configurations and good tissue/cell morphology. Immunohistochemistry and in situ hybridization study also revealed well-preserved proteins and mRNAs on TMA slides. Our method significantly simplifies TMA preparation and assembly when frozen pathological tissues are used. Our technology provides an alternative tool for creating high-quality TMAs for the general research community to study gene expressions in pathological samples. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1940-9818 0736-6205 |
| Relation: | https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818 |
| DOI: | 10.2144/000112498 |
| Access URL: | https://doaj.org/article/2679821bc5f34582bf517393cffb1d5e |
| Accession Number: | edsdoj.2679821bc5f34582bf517393cffb1d5e |
| Database: | Directory of Open Access Journals |
| ISSN: | 19409818 07366205 |
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| DOI: | 10.2144/000112498 |
| Published in: | BioTechniques |
| Language: | English |