Academic Journal
Polyoxazoline-conjugated porcine serum albumin as an artificial plasma expander for dogs
| Title: | Polyoxazoline-conjugated porcine serum albumin as an artificial plasma expander for dogs |
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| Authors: | Wataru Okamoto, Tomone Usui, Mai Hasegawa, Tatsuhiro Kobayashi, Junya Fujisawa, Kazuaki Taguchi, Kazuaki Matsumoto, Mitsutomo Kohno, Masayuki Iwazaki, Shotaro Shimano, Itsuma Nagao, Hiroto Toyoda, Naoki Matsumura, Hirotaka Tomiyasu, Ryota Tochinai, Teruyuki Komatsu |
| Source: | Scientific Reports, Vol 13, Iss 1, Pp 1-15 (2023) |
| Publisher Information: | Nature Portfolio, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Medicine, Science |
| More Details: | Abstract Veterinary medicine has made tremendous progress for domestic dogs, which are irreplaceable family members enriching human life. Nevertheless, no adequate supply system exists for their blood products. This study examined the synthesis, structure, safety, and efficacy of poly(2-ethyl-2-oxazoline)-conjugated porcine serum albumin (POx-PSA) as an artificial plasma expander for dogs. The aqueous POx-PSA solution showed moderately high colloid osmotic pressure and good blood cell compatibility. Actually, lyophilized powder stored for 1 year can regenerate into a homogeneous solution. The circulation half-life of POx-PSA in rats was 2.1-fold longer than that of naked PSA. Rats produced neither anti-PSA IgG antibody nor anti-POx IgG antibody, which suggests excellent immunological stealth properties of POx-PSA. Complete resuscitation of hemorrhagic shock in rats was achieved soon after injection of POx-PSA solution. Serum biochemistry tests and histopathological observations indicated no abnormality in the related organs. When POx-PSA was administered to dogs intravenously, (i) no serum biochemical or hematological alteration was observed, also (ii) no overt deterioration of animal health was observed. These results indicate that POx-PSA has potential as an artificial plasma expander for dogs. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-023-35999-4 |
| Access URL: | https://doaj.org/article/e265ef0d2d004cd0bc6c462a06f60241 |
| Accession Number: | edsdoj.265ef0d2d004cd0bc6c462a06f60241 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20452322 |
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| DOI: | 10.1038/s41598-023-35999-4 |
| Published in: | Scientific Reports |
| Language: | English |