Bibliographic Details
| Title: |
Persistent human bocavirus 1 infection and tonsillar immune responses |
| Authors: |
Lotta E. Ivaska, Antti Silvoniemi, Oscar Palomares, Riitta Turunen, Matti Waris, Emilia Mikola, Tuomo Puhakka, Maria Söderlund‐Venermo, Mübeccel Akdis, Cezmi A. Akdis, Tuomas Jartti |
| Source: |
Clinical and Translational Allergy, Vol 11, Iss 6, Pp n/a-n/a (2021) |
| Publisher Information: |
Wiley, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Immunologic diseases. Allergy |
| Subject Terms: |
cytokine, forkheadbox protein 3, FOXP3, human bocavirus, RAR‐related orphan receptor C 2, RORC2, Immunologic diseases. Allergy, RC581-607 |
| More Details: |
Abstract Background Persistent human bocavirus 1 (HBoV1) infection is a common finding in patients suffering from chronic tonsillar disease. However, the associations between HBoV1 infection and specific immune reactions are not completely known. We aimed to compare in vivo expression of T‐cell cytokines, transcription factors, and type I/III interferons in human tonsils between HBoV1‐positive and ‐negative tonsillectomy patients. Methods Tonsil tissue samples, nasopharyngeal aspirate (NPA), and serum samples were obtained from 143 immunocompetent adult and child tonsillectomy patients. HBoV1 and 14 other respiratory viruses were detected in NPAs and tonsil tissues by polymerase chain reaction (PCR). Serology and semi‐quantitative PCR were used for diagnosing HBoV1 infections. Expression of 14 cytokines and transcription factors (IFN‐α, IFN‐β, IFN‐γ, IL‐10, IL‐13, IL‐17, IL‐28, IL‐29, IL‐37, TGF‐β, FOXP3, GATA3, RORC2, Tbet) was analyzed by quantitative reverse‐transcription (RT)‐PCR in tonsil tissues. Results HBoV1 was detected by PCR in NPA and tonsils from 25 (17%) study patients. Serology results indicated prior nonacute infections in 81% of cases. Tonsillar cytokine responses were affected by HBoV1 infection. The suppression of two transcription factors, RORC2 and FOXP3, was associated with HBoV1 infection (p |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2045-7022 |
| Relation: |
https://doaj.org/toc/2045-7022 |
| DOI: |
10.1002/clt2.12030 |
| Access URL: |
https://doaj.org/article/2632f69d71a64e3fbe951aca326e21f2 |
| Accession Number: |
edsdoj.2632f69d71a64e3fbe951aca326e21f2 |
| Database: |
Directory of Open Access Journals |
