Academic Journal
Comprehensive proteomics and functional annotation of mouse brown adipose tissue.
| Title: | Comprehensive proteomics and functional annotation of mouse brown adipose tissue. |
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| Authors: | Jing Li, Juan Li, Wei-Gang Zhao, Hai-Dan Sun, Zheng-Guang Guo, Xiao-Yan Liu, Xiao-Yue Tang, Zhu-Fang She, Tao Yuan, Shuai-Nan Liu, Quan Liu, Yong Fu, Wei Sun |
| Source: | PLoS ONE, Vol 15, Iss 5, p e0232084 (2020) |
| Publisher Information: | Public Library of Science (PLoS), 2020. |
| Publication Year: | 2020 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Medicine, Science |
| More Details: | Knowledge about the mouse brown adipose tissue (BAT) proteome can provide a deeper understanding of the function of mammalian BAT. Herein, a comprehensive analysis of interscapular BAT from C57BL/6J female mice was conducted by 2DLC and high-resolution mass spectrometry to construct a comprehensive proteome dataset of mouse BAT proteins. A total of 4949 nonredundant proteins were identified, and 4495 were quantified using the iBAQ method. According to the iBAQ values, the BAT proteome was divided into high-, middle- and low-abundance proteins. The functions of the high-abundance proteins were mainly related to glucose and fatty acid oxidation to produce heat for thermoregulation, while the functions of the middle- and low-abundance proteins were mainly related to protein synthesis and apoptosis, respectively. Additionally, 497 proteins were predicted to have signal peptides using SignalP4 software, and 75 were confirmed in previous studies. This study, for the first time, comprehensively profiled and functionally annotated the BAT proteome. This study will be helpful for future studies focused on biomarker identification and BAT molecular mechanisms. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1932-6203 |
| Relation: | https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0232084 |
| Access URL: | https://doaj.org/article/256b8764b84045e9b40d45e91ce17585 |
| Accession Number: | edsdoj.256b8764b84045e9b40d45e91ce17585 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 19326203 |
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| DOI: | 10.1371/journal.pone.0232084 |
| Published in: | PLoS ONE |
| Language: | English |