Academic Journal
Fully closed cell sorter for immune cell therapy manufacturing
| Title: | Fully closed cell sorter for immune cell therapy manufacturing |
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| Authors: | Masahiro Matsumoto, Shinji Tashiro, Tatsumi Ito, Kazuya Takahashi, Gakuji Hashimoto, Junji Kajihara, Yoshihiro Miyahara, Hiroshi Shiku, Yoichi Katsumoto |
| Source: | Molecular Therapy: Methods & Clinical Development, Vol 30, Iss , Pp 367-376 (2023) |
| Publisher Information: | Elsevier, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Genetics LCC:Cytology |
| Subject Terms: | cell therapy manufacturing, cell sorting, cell purification, functional assay, cGMP, cell therapy, Genetics, QH426-470, Cytology, QH573-671 |
| More Details: | By analyzing patients treated with adoptive immune cell therapies, various immune cell phenotypes have been found in the starting and infused materials as determinants of sustained remission. The isolation of these specific phenotypes for clinical use requires current Good Manufacturing Practice (cGMP)-compliant cell-sorting technologies with multiparameter selection capabilities. Here, we developed a cGMP-requirement-applicable fully closed cell sorter that has a suction mechanism and multiparameter detection using two laser optical settings. Negative pressure generated by a change in the chamber volume at a sorting point allows the isolation of cells of interest with high viability and purity. Our study demonstrated that this microfluidic sorter enables the isolation of cells of interest at an effective rate of 7,000 sorts per second on average. A purity of 85.5% and 77.1% effective yield with 93.7% viability was obtained when applying a target population of 35.9% in total (lymphocyte+CD8+) at 15,000 events per second (2 × 107 cells/mL). The sorted gene-modified T cells maintain largely unaltered proliferation, antigen recognition, cytokine release, and cytotoxicity functionalities. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2329-0501 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2329050123001146; https://doaj.org/toc/2329-0501 |
| DOI: | 10.1016/j.omtm.2023.07.012 |
| Access URL: | https://doaj.org/article/2519a81bb32f4c479886bbd35c498d85 |
| Accession Number: | edsdoj.2519a81bb32f4c479886bbd35c498d85 |
| Database: | Directory of Open Access Journals |
| ISSN: | 23290501 |
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| DOI: | 10.1016/j.omtm.2023.07.012 |
| Published in: | Molecular Therapy: Methods & Clinical Development |
| Language: | English |