Simultaneous Detection of Barley Virus Diseases in Korea
| Title: | Simultaneous Detection of Barley Virus Diseases in Korea |
|---|---|
| Authors: | Bong-Choon Lee, Ju-Young Bae, Sang-Min Kim, Ji-Eun Ra, Nak Jung Choi, Man Young Choi, Ki Do Park |
| Source: | Research in Plant Disease, Vol 23, Iss 4, Pp 363-366 (2017) |
| Publisher Information: | Hanrimwon Publishing Company, 2017. |
| Publication Year: | 2017 |
| Collection: | LCC:Agriculture (General) |
| Subject Terms: | Barley mild mosaic virus, Barley yellow mosaic virus, Barley yellow dwarf virus, mRT-PCR, Simultaneous detection, Agriculture (General), S1-972 |
| More Details: | Barley mild mosaic virus (BaMMV), Barley yellow mosaic virus (BaYMV) and Barley yellow dwarf virus (BYDV) have been identified as an important causative agents for an economically important disease of winter barley in Korea. In this study, a multiplex reverse transcription polymerase chain reaction (mRT-PCR) method was used for the simultaneous detection. Three sets of virus-specific primers targeted to the capsid protein coding genes of BaMMV, BaYMV and BYDV were used to amplify fragments that were 594 bp, 461 bp, and 290 bp, respectively. Several sets of primers for each target virus were evaluated for their sensitivity and specificity by multiplex RT-PCR. The optimum primer concentrations and RT-PCR conditions were determined for the multiplex RT-PCR. The mRT-PCR assay was found to be a better and rapid virus diagnostic tool of specific barley diseases and potential for investigating the epidemiology of these viral diseases. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English Korean |
| ISSN: | 1598-2262 |
| Relation: | http://www.online-rpd.org/journal/view.html?doi=10.5423/RPD.2017.23.4.363; https://doaj.org/toc/1598-2262 |
| DOI: | 10.5423/RPD.2017.23.4.363 |
| Access URL: | https://doaj.org/article/24799a4c10894005bc2ecbaf55766388 |
| Accession Number: | edsdoj.24799a4c10894005bc2ecbaf55766388 |
| Database: | Directory of Open Access Journals |
| ISSN: | 15982262 |
|---|---|
| DOI: | 10.5423/RPD.2017.23.4.363 |
| Published in: | Research in Plant Disease |
| Language: | English Korean |