Bibliographic Details
| Title: |
Specific changes in amino acid profiles in monocytes of patients with breast, lung, colorectal and ovarian cancers |
| Authors: |
Vitaliy Chagovets, Natalia Starodubtseva, Alisa Tokareva, Anastasia Novoselova, Marina Patysheva, Irina Larionova, Elizaveta Prostakishina, Militsa Rakina, Anna Kazakova, Evgenii Topolnitskiy, Nikolay Shefer, Julia Kzhyshkowska, Vladimir Frankevich, Gennadiy Sukhikh |
| Source: |
Frontiers in Immunology, Vol 14 (2024) |
| Publisher Information: |
Frontiers Media S.A., 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Immunologic diseases. Allergy |
| Subject Terms: |
mass spectrometry, metabolomics, oncology, monocytes, tumor-associated macrophages, Immunologic diseases. Allergy, RC581-607 |
| More Details: |
IntroductionImmunometabolism is essential factor of tumor progression, and tumor-associated macrophages are characterized by substantial changes in their metabolic status. In this study for the first time, we applied targeted amino acid LC-MS/MS analysis to compare amino acid metabolism of circulating monocytes isolated from patients with breast, ovarian, lung, and colorectal cancer.MethodsMonocyte metabolomics was analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/ MS) analysis of amino acid extracts. The targeted analysis of 26 amino acids was conducted by LCMS/MS on an Agilent 6460 triple quadrupole mass spectrometer equipped with an electrospray ionization source and an Agilent 1260 II liquid chromatograph.ResultsComparison of monocytes of cancer patients with monocytes of healthy control individuals demonstrated that in breast cancer most pronounced changes were identified for tryptophan (AUC = 0.76); for ovarian cancer, aminobutyric acid was significantly elevated (AUC= 1.00); for lung cancer significant changes we indented for citrulline (AUC = 0.70). In order to identify key amino acids that are characteristic for monocytes in specific cancer types, we compared each individual cancer with other 3 types of cancer. We found, that aspartic acid and citrulline are specific for monocytes of patients with colorectal cancer (p |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-3224 |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fimmu.2023.1332043/full; https://doaj.org/toc/1664-3224 |
| DOI: |
10.3389/fimmu.2023.1332043 |
| Access URL: |
https://doaj.org/article/246565c522cc4e35a3507cd87442b9f4 |
| Accession Number: |
edsdoj.246565c522cc4e35a3507cd87442b9f4 |
| Database: |
Directory of Open Access Journals |
