Bibliographic Details
| Title: |
The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells |
| Authors: |
Rajiv Sharma, Daniel P. Dever, Ciaran M. Lee, Armon Azizi, Yidan Pan, Joab Camarena, Thomas Köhnke, Gang Bao, Matthew H. Porteus, Ravindra Majeti |
| Source: |
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021) |
| Publisher Information: |
Nature Portfolio, 2021. |
| Publication Year: |
2021 |
| Collection: |
LCC:Science |
| Subject Terms: |
Science |
| More Details: |
Genetic barcoding has been used to track clonal dynamics of cells. Here, the authors develop a Tracking Recombination Alleles in Clonal Engraftment using sequencing (TRACE-Seq), to barcode repaired alleles by introducing silent mutations or outside of coding regions, to show clonal complexity of edited CD34 + cells following engraftment. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2041-1723 |
| Relation: |
https://doaj.org/toc/2041-1723 |
| DOI: |
10.1038/s41467-020-20792-y |
| Access URL: |
https://doaj.org/article/24227f1bebe84818bfcc9151aa481b5d |
| Accession Number: |
edsdoj.24227f1bebe84818bfcc9151aa481b5d |
| Database: |
Directory of Open Access Journals |
