Bibliographic Details
| Title: |
Comprehensive benchmarking of CITE-seq versus DOGMA-seq single cell multimodal omics |
| Authors: |
Zhongli Xu, Elisa Heidrich-O’Hare, Wei Chen, Richard H. Duerr |
| Source: |
Genome Biology, Vol 23, Iss 1, Pp 1-17 (2022) |
| Publisher Information: |
BMC, 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Biology (General)
LCC:Genetics |
| Subject Terms: |
Biology (General), QH301-705.5, Genetics, QH426-470 |
| More Details: |
Abstract The recently developed method TEA-seq and similar DOGMA-seq single cell trimodal omics assays provide unprecedented opportunities for understanding cell biology, but independent evaluation is lacking. We explore the utility of DOGMA-seq compared to the bimodal CITE-seq assay in activated and stimulated human peripheral blood T cells. We find that single cell trimodal omics measurements after digitonin (DIG) permeabilization were generally better than after an alternative “low-loss lysis” (LLL) permeabilization condition. Next, we find that DOGMA-seq with optimized DIG permeabilization and its ATAC library provides more information, although its mRNA and cell surface protein libraries have slightly inferior quality, compared to CITE-seq. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1474-760X |
| Relation: |
https://doaj.org/toc/1474-760X |
| DOI: |
10.1186/s13059-022-02698-8 |
| Access URL: |
https://doaj.org/article/2351dac33ab24303aa1d884709507686 |
| Accession Number: |
edsdoj.2351dac33ab24303aa1d884709507686 |
| Database: |
Directory of Open Access Journals |
