Bibliographic Details
| Title: |
Identification of the interacting proteins of Bambusa pervariabilis × Dendrocalamopsis grandis in response to the transcription factor ApCtf1β in Arthrinium phaeospermum |
| Authors: |
Peng Yan, Jiawen Yu, Xinmei Fang, Shuying Li, Shan Han, Tiantian Lin, Yinggao Liu, Chunlin Yang, Fang He, Tianhui Zhu, Shujiang Li |
| Source: |
Frontiers in Plant Science, Vol 13 (2022) |
| Publisher Information: |
Frontiers Media S.A., 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Plant culture |
| Subject Terms: |
Arthrinium phaeospermum, ApCtf1β, Y2H, BiFC, GST pull-down, qRT-PCR, Plant culture, SB1-1110 |
| More Details: |
Arthrinium phaeospermum is the main pathogen that causes Bambusa pervariabilis × Dendrocalamopsis grandis blight. It secretes the cutinase transcription factor ApCtf1β, which has been shown to play an important role in B. pervariabilis × D. grandis virulence. However, knowledge about the interaction target genes of ApCtf1β in B. pervariabilis × D. grandis remains limited. A cDNA library for the yeast two-hybrid system was constructed from B. pervariabilis × D. grandis shoots after 168 h treatment with A. phaeospermum. The library was identified as 1.20 × 107 cfu, with an average insert >1,000 bp in size and a 100% positive rate, providing a database for the subsequent molecular study of the interaction between A. phaeospermum and B. pervariabilis × D. grandis. The yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC), and glutathione-S-transferase (GST) pull-down assays were used to screen for and identify two ApCtf1β interacting target proteins, BDUbc and BDSKL1, providing a reliable theoretical basis to study the molecular mechanism underlying B. pervariabilis × D. grandis resistance in response to A. phaeospermum, which would, in turn, establish a platform to develop new strategies for the sustainable and effective control of the blight diseases of forest trees. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-462X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fpls.2022.991077/full; https://doaj.org/toc/1664-462X |
| DOI: |
10.3389/fpls.2022.991077 |
| Access URL: |
https://doaj.org/article/2304b52eb75f4c49b81d0f3c136bf4f7 |
| Accession Number: |
edsdoj.2304b52eb75f4c49b81d0f3c136bf4f7 |
| Database: |
Directory of Open Access Journals |
