Academic Journal
The development of a novel zeolite-based assay for efficient and deep plasma proteomic profiling
| Title: | The development of a novel zeolite-based assay for efficient and deep plasma proteomic profiling |
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| Authors: | Nan Li, Jingnan Huang, Shangwen He, Qiaocong Zheng, Feng Ye, Zhengxing Qin, Dong Wang, Ting Xiao, Mengyuan Mao, Zhenhua Zhou, Tingxi Tang, Longshan Zhang, Xiaoqing Wang, Yingqiao Wang, Ying Lyu, Laiyu Liu, Lingyun Dai, Jigang Wang, Jian Guan |
| Source: | Journal of Nanobiotechnology, Vol 22, Iss 1, Pp 1-23 (2024) |
| Publisher Information: | BMC, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Biotechnology LCC:Medical technology |
| Subject Terms: | Plasma, Proteomics, Zeolite, Protein corona, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5 |
| More Details: | Abstract Plasma proteins are considered the most informative source of biomarkers for disease diagnosis and monitoring. Mass spectrometry (MS)-based proteomics has been applied to identify biomarkers in plasma, but the complexity of the plasma proteome and the extremely large dynamic range of protein abundances in plasma make the clinical application of plasma proteomics highly challenging. We designed and synthesized zeolite-based nanoparticles to deplete high-abundance plasma proteins. The resulting novel plasma proteomic assay can measure approximately 3000 plasma proteins in a 45 min chromatographic gradient. Compared to those in neat and depleted plasma, the plasma proteins identified by our assay exhibited distinct biological profiles, as validated in several public datasets. A pilot investigation of the proteomic profile of a hepatocellular carcinoma (HCC) cohort identified 15 promising protein features, highlighting the diagnostic value of the plasma proteome in distinguishing individuals with and without HCC. Furthermore, this assay can be easily integrated with all current downstream protein profiling methods and potentially extended to other biofluids. In conclusion, we established a robust and efficient plasma proteomic assay with unprecedented identification depth, paving the way for the translation of plasma proteomics into clinical applications. Graphical Abstract |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1477-3155 |
| Relation: | https://doaj.org/toc/1477-3155 |
| DOI: | 10.1186/s12951-024-02404-9 |
| Access URL: | https://doaj.org/article/22666f4f031b42e5a3fc814ca013083f |
| Accession Number: | edsdoj.22666f4f031b42e5a3fc814ca013083f |
| Database: | Directory of Open Access Journals |
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| ISSN: | 14773155 |
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| DOI: | 10.1186/s12951-024-02404-9 |
| Published in: | Journal of Nanobiotechnology |
| Language: | English |