Diagnostic performance of the indirect immunofluorescence test for the detection of antibodies against HTLV-1
| Title: | Diagnostic performance of the indirect immunofluorescence test for the detection of antibodies against HTLV-1 |
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| Authors: | Soledad Romero-Ruiz, Eduardo Miranda-Ulloa E., Ronal Briceño-Espinoza |
| Source: | Revista Peruana de Medicina Experimental y Salud Pública, Vol 34, Iss 3, Pp 459-65 (2017) |
| Publisher Information: | Instituto Nacional de Salud, 2017. |
| Publication Year: | 2017 |
| Collection: | LCC:Medicine LCC:Medicine (General) |
| Subject Terms: | virus 1 linfotrópico t humano, diagnóstico, técnica del anticuerpo fluorescente indirecta, Medicine, Medicine (General), R5-920 |
| More Details: | The objective of the study was to determine the diagnostic yield of the indirect immunofluorescence (IFI) test for the detection of antibodies against HTLV-1. A diagnostic test evaluation study was performed. HTLV-1-infected MT2 cells and HTLV-1-uninfected K-562 cells were cultured; then these cells were impregnated and fixed in sheets for immunofluorescence and faced to Peruvian sera. A total of 155 sera (80 HTLV-1-positive sera and 75 sera positive for other diseases) from the Peruvian Instituto Nacional de Salud were used. In addition, the parameters of repeatability (intra-laboratory) and reproducibility (in laboratories of the Peruvian coast, mountains and jungle) of the test were evaluated. The IFI test detected the presence of antibodies against HTLV-1 reaching a sensitivity of 98.75% (95% CI: 95.69 – 100.00%), a specificity of 98.67% (95% CI: 95.40 – 100.00%) and the Kappa index was 0.975. There were no false positives or false negatives; however, one undetermined result and one non-specific result were obtained. The test showed 100% qualitative agreement when performing the repeatability and reproducibility. The results obtained are comparable to the reference test. Therefore, the IFI test had a good diagnostic performance and would be useful for the confirmation of HTLV-1. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | Spanish; Castilian |
| ISSN: | 1726-4634 1726-4642 |
| Relation: | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2635; https://doaj.org/toc/1726-4634; https://doaj.org/toc/1726-4642 |
| DOI: | 10.17843/rpmesp.2017.343.2635 |
| Access URL: | https://doaj.org/article/21bc7ff045dc47aeb661090c645b38e5 |
| Accession Number: | edsdoj.21bc7ff045dc47aeb661090c645b38e5 |
| Database: | Directory of Open Access Journals |
| ISSN: | 17264634 17264642 |
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| DOI: | 10.17843/rpmesp.2017.343.2635 |
| Published in: | Revista Peruana de Medicina Experimental y Salud Pública |
| Language: | Spanish; Castilian |