Academic Journal
Fibroblast transition to an endothelial 'trans' state improves cell reprogramming efficiency
| Title: | Fibroblast transition to an endothelial 'trans' state improves cell reprogramming efficiency |
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| Authors: | Megumi Mathison, Deepthi Sanagasetti, Vivek P. Singh, Aarthi Pugazenthi, Jaya Pratap Pinnamaneni, Christopher T. Ryan, Jianchang Yang, Todd K. Rosengart |
| Source: | Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| Publisher Information: | Nature Portfolio, 2021. |
| Publication Year: | 2021 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Medicine, Science |
| More Details: | Abstract Fibroblast reprogramming offers the potential for myocardial regeneration via in situ cell transdifferentiation. We explored a novel strategy leveraging endothelial cell plasticity to enhance reprogramming efficiency. Rat cardiac endothelial cells and fibroblasts were treated with Gata4, Mef2c, and Tbx5 (GMT) to assess the cardio-differentiation potential of these cells. The endothelial cell transdifferentiation factor ETV2 was transiently over-expressed in fibroblasts followed by GMT treatment to assess “trans-endothelial” cardio-differentiation. Endothelial cells treated with GMT generated more cTnT+ cells than did cardiac fibroblasts (13% ± 2% vs 4% ± 0.5%, p |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-021-02056-x |
| Access URL: | https://doaj.org/article/dd218c69e5e94d91a0439e753722da4a |
| Accession Number: | edsdoj.218c69e5e94d91a0439e753722da4a |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20452322 |
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| DOI: | 10.1038/s41598-021-02056-x |
| Published in: | Scientific Reports |
| Language: | English |