Academic Journal
Analysis of fecal bile acids and metabolites by high resolution mass spectrometry in farm animals and correlation with microbiota
| Title: | Analysis of fecal bile acids and metabolites by high resolution mass spectrometry in farm animals and correlation with microbiota |
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| Authors: | Emanuele Porru, Daniel Scicchitano, Nicolò Interino, Teresa Tavella, Marco Candela, Aldo Roda, Jessica Fiori |
| Source: | Scientific Reports, Vol 12, Iss 1, Pp 1-15 (2022) |
| Publisher Information: | Nature Portfolio, 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Medicine LCC:Science |
| Subject Terms: | Medicine, Science |
| More Details: | Abstract There is a growing interest in the named “acidic sterolbiome” and in the genetic potential of the gut microbiome (GM) to modify bile acid (BA) structure. Indeed, the qualitative composition of BAs in feces correlates with the bowel microorganisms and their collective genetic material. GM is responsible for the production of BA metabolites, such as secondary and oxo-BAs. The specific BA profiles, as microbiome-host co-metabolic products, could be useful to investigate the GM-host interaction in animals under physiological conditions, as well as in specific diseases. In this context, we developed and validated an ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method for the simultaneous analysis of up to 21 oxo-BAs and their 9 metabolic precursors. Chromatographic separation was achieved in 7 min with adequate analytical performance in terms of selectivity, sensitivity (LOQ from 0.05 to 0.1 µg/mL), accuracy (bias% 90%. In parallel, the gut microbiota assessment in farming animals was evaluated by 16S rRNA next-generation sequencing, and the correlation with the BA composition was performed by multivariate analysis, allowing to reconstruct species-specific associations between the BA profile and specific GM components. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-022-06692-9 |
| Access URL: | https://doaj.org/article/204177e42d78489c8267f81e6af77b92 |
| Accession Number: | edsdoj.204177e42d78489c8267f81e6af77b92 |
| Database: | Directory of Open Access Journals |
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| ISSN: | 20452322 |
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| DOI: | 10.1038/s41598-022-06692-9 |
| Published in: | Scientific Reports |
| Language: | English |