First report of free-living amoebae in watercourses in southern Brazil: molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp.
| Title: | First report of free-living amoebae in watercourses in southern Brazil: molecular diagnosis and phylogenetic analysis of Vermamoeba vermiformis, Naegleria gruberi, and Acanthamoeba spp. |
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| Authors: | Brenda Teixeira Scardini Marinho, Denise Leal dos Santos, Daniel Leal dos Santos, Marilise Brittes Rott |
| Source: | Journal of Water and Health, Vol 21, Iss 7, Pp 972-980 (2023) |
| Publisher Information: | IWA Publishing, 2023. |
| Publication Year: | 2023 |
| Collection: | LCC:Public aspects of medicine |
| Subject Terms: | acanthamoeba spp., dilúvio stream, free-living amoebae, guaíba lake, naegleria gruberi, vermamoeba vermiformis, Public aspects of medicine, RA1-1270 |
| More Details: | Free-living amoebae (FLA) are protozoa dispersed in different environments and are responsible for different infections caused to humans and other animals. Microorganisms such as Acanthamoeba spp., Vermamoeba sp., and Naegleria sp. are associated with diseases that affect the central nervous system, in addition to skin infections and keratitis, as occurs in the genus Acanthamoeba and with Vermamoeba vermiformis. Due to the concerns of these FLA in anthropogenic aquatic environments, this work aimed to identify these microorganisms present in waters of Porto Alegre, Brazil. One litre sample was collected in two watercourses during the summer of 2022 and inoculated onto non-nutrient agar plates containing heat-inactivated Escherichia coli. Polymerase chain reaction results indicated the presence of FLA of the genera Acanthamoeba, Vermamoeba, and Naegleria in the study areas. Genetic sequencing indicated the presence of V. vermiformis and Naegleria gruberi. These aquatic and anthropogenic environments can serve as a means of spread and contamination by FLA, which gives valuable information on public health in the city. HIGHLIGHTS Identify microorganisms present in anthropogenic aquatic environments.; First report of free-living amoebae in southern Brazil.; Detection of Acanthamoeba spp., Naegleria gruberi, and Vermamoeba vermiformis in watercourses.; These microorganisms are responsible for causing fatal diseases that affect the central nervous system.; These environments can serve as a means of spread and contamination.; |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 1477-8920 1996-7829 |
| Relation: | http://jwh.iwaponline.com/content/21/7/972; https://doaj.org/toc/1477-8920; https://doaj.org/toc/1996-7829 |
| DOI: | 10.2166/wh.2023.126 |
| Access URL: | https://doaj.org/article/1f8eb30203224a708fd3b148f4dfdebd |
| Accession Number: | edsdoj.1f8eb30203224a708fd3b148f4dfdebd |
| Database: | Directory of Open Access Journals |
| ISSN: | 14778920 19967829 |
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| DOI: | 10.2166/wh.2023.126 |
| Published in: | Journal of Water and Health |
| Language: | English |