| Title: |
Establishment of enzyme-linked immunosorbent assay for beef and lamb contents in cooked meat |
| Authors: |
Yujing Li, Jingjing Liu, Sufang Fan, Zhao Li, Jing Zhang, Erjing Zhang, Ziran Li, Yan Zhang, Chunsheng Li |
| Source: |
Journal of Future Foods, Vol 4, Iss 1, Pp 91-96 (2024) |
| Publisher Information: |
KeAi Communications Co. Ltd., 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Food processing and manufacture |
| Subject Terms: |
Double antibody sandwich enzyme-linked immunosorbent assay (ELISA), Beef components, Lamb components, Food processing and manufacture, TP368-456 |
| More Details: |
In this study, an enzyme-linked immunosorbent assay (ELISA) was established to detect beef and lamb components, and its performance was tested. Double-antibody sandwich ELISA was adopted and determined a coating concentration of capture antibody 3G5 of 1:4 000, a working concentration of enzyme-labeled antibody 2E7-horseradish peroxidase (HRP) of 1:1 000, a sample incubation time of 60 min and a detection antibody reaction time of 60 min. The specificity, sensitivity, repeatability and stability of this assay were determined. The limit of detection for beef and lamb skeletal muscle troponin I was 45 mg/kg, the inter-assay and intra-assay recovery rates ranged from 80.4% to 115.7%, the coefficients of variation were below 13.6%, and the cross reaction rates of the tissue components of chicken, duck and fish were below 13.4%. The sandwich ELISA method established in this study is stable and has high accuracy. The test results were consistent with the polymerase chain reaction (PCR) method at 50 and 100 g/kg. Therefore, this ELISA method can be used to quantitatively detect beef and lamb components in meat products. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2772-5669 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2772566923000435; https://doaj.org/toc/2772-5669 |
| DOI: |
10.1016/j.jfutfo.2023.05.010 |
| Access URL: |
https://doaj.org/article/1f0502d84d1545168e45f59a87814e60 |
| Accession Number: |
edsdoj.1f0502d84d1545168e45f59a87814e60 |
| Database: |
Directory of Open Access Journals |
