| Title: |
Combination of percutaneous thermal ablation and adoptive Th9 cell transfer therapy against non-small cell lung cancer |
| Authors: |
Hanbo Pan, Yu Tian, Siyu Pei, Wanlin Yang, Yanyang Zhang, Zenan Gu, Hongda Zhu, Ningyuan Zou, Jiaqi Zhang, Long Jiang, Yingjie Hu, Shengping Shen, Kai Wang, Haizhen Jin, Ziming Li, Yanyun Zhang, Yichuan Xiao, Qingquan Luo, Hui Wang, Jia Huang |
| Source: |
Experimental Hematology & Oncology, Vol 13, Iss 1, Pp 1-24 (2024) |
| Publisher Information: |
BMC, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Diseases of the blood and blood-forming organs
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| Subject Terms: |
Non-small cell lung cancer, Percutaneous thermal ablation, Adoptive Th9 cell therapy, Tumor immunotherapy, Patient-derived xenograft, Diseases of the blood and blood-forming organs, RC633-647.5, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| More Details: |
Abstract Background Non-small cell lung cancer (NSCLC) is one of the predominant malignancies globally. Percutaneous thermal ablation (PTA) has gained widespread use among NSCLC patients, with the potential to elicit immune responses but limited therapeutic efficacies for advanced-stage disease. T-helper type 9 (Th9) cells are a subset of CD4+ effector T cells with robust and persistent anti-tumor effects. This study proposes to develop PTA-Th9 cell integrated therapy as a potential strategy for NSCLC treatment. Methods The therapeutic efficacies were measured in mice models with subcutaneously transplanted, recurrence, or lung metastatic tumors. The tumor microenvironments (TMEs) were evaluated by flow cytometry. The cytokine levels were assessed by ELISA. The signaling molecules were determined by quantitative PCR and Western blotting. The translational potential was tested in the humanized NSCLC patient-derived xenograft (PDX) model. Results We find that PTA combined with adoptive Th9 cell transfer therapy substantially suppresses tumor growth, recurrence, and lung metastasis, ultimately extending the survival of mice with NSCLC grafts, outperforming both PTA and Th9 cell transfer monotherapy. Analysis of TMEs indicates that combinatorial therapy significantly augments tumor-infiltrating Th9 cells, boosts anti-tumor effects of CD8+ T cells, and remodels tumor immunosuppressive microenvironments. Moreover, combinatorial therapy significantly strengthens the regional and circulation immune response of CD8+ T cells in mice with tumor lung metastasis and induces peripheral CD8+ T effector memory cells in mice with tumor recurrence. Mechanically, PTA reinforces the anti-tumor ability of Th9 cells primarily through upregulating interleukin (IL)-1β and subsequently activating the downstream STAT1/IRF1 pathway, which could be effectively blocked by intercepting IL-1β signaling. Finally, the enhanced therapeutic effect of combinatorial therapy is validated in humanized NSCLC PDX models. Conclusions Collectively, this study demonstrates that combinatorial therapy displays robust and durable anti-tumor efficacy and excellent translational potential, offering excellent prospects for translation and emerging as a promising approach for NSCLC treatment. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2162-3619 |
| Relation: |
https://doaj.org/toc/2162-3619 |
| DOI: |
10.1186/s40164-024-00520-8 |
| Access URL: |
https://doaj.org/article/c1ea960d84b14322a3389936d2f41a36 |
| Accession Number: |
edsdoj.1ea960d84b14322a3389936d2f41a36 |
| Database: |
Directory of Open Access Journals |
