| Title: |
Photoactive metabolite mediated photodynamic therapy of Rhabdomyosarcoma cell lines using medicinal plants and Doxorubicin co-treatments |
| Authors: |
Sumbal Javaid, Irfan Zia Qureshi, Ahmat Khurshid, Tayyaba Afsar, Fohad Mabood Husain, Muhammad Khurshid, Janeen H. Trembley, Suhail Razak |
| Source: |
BMC Complementary Medicine and Therapies, Vol 24, Iss 1, Pp 1-13 (2024) |
| Publisher Information: |
BMC, 2024. |
| Publication Year: |
2024 |
| Collection: |
LCC:Other systems of medicine |
| Subject Terms: |
Photosensitizer, Rhabdomyosarcoma cancer, Photodynamic therapy, Absorption spectroscopy, Combination index, Medicinal plants, Other systems of medicine, RZ201-999 |
| More Details: |
Abstract Background Medicinal plant-mediated combinational therapies have gained importance globally due to minimal side effects and enhanced treatment outcomes compared to single-drug modalities. We aimed to analyze the cytotoxic potential of each conventional treatment i.e., photodynamic therapy (PDT), chemotherapy (doxorubicin hydrochloride; Dox-HCl) with or without various concentrations of medicinal plant extracts (PE) on soft tissue cancer Rhabdomyosarcoma (RD) cell line. Methods The Rhabdomyosarcoma (RD) cell line was cultured and treated with Photosensitizer (Photosense (AlPc4)), Chemo (Dox-HCl), and their combinations with different concentrations of each plant extract i.e., Thuja occidentalis, Moringa oleifera, Solanum surattense. For the source of illumination, a Diode laser (λ = 630 nm ± 1 nm, Pmax = 1.5 mW) was used. Photosensitizer uptake time (∼ 45 min) was optimized through spectrophotometric measurements (absorption spectroscopy). Drug response of each treatment arm was assessed post 24 h of administration using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5- 5-diphenyl-2 H- tetrazolium bromide (MTT) assay. Results PE-mediated Chemo-Photodynamic therapy (PDT) exhibited synergistic effects (CI |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2662-7671 |
| Relation: |
https://doaj.org/toc/2662-7671 |
| DOI: |
10.1186/s12906-024-04575-2 |
| Access URL: |
https://doaj.org/article/d1e296fc6b864a9e868c179ea2ce22b0 |
| Accession Number: |
edsdoj.1e296fc6b864a9e868c179ea2ce22b0 |
| Database: |
Directory of Open Access Journals |
