Structural Characterization, Antimicrobial, Antibiofilm, Antioxidant, Anticancer and Acute Toxicity Properties of N-(2-hydroxyphenyl)-2-phenazinamine From Nocardiopsis exhalans (KP149558)
| Title: | Structural Characterization, Antimicrobial, Antibiofilm, Antioxidant, Anticancer and Acute Toxicity Properties of N-(2-hydroxyphenyl)-2-phenazinamine From Nocardiopsis exhalans (KP149558) |
|---|---|
| Authors: | Vaikundamoorthy Ramalingam, Rajendran Rajaram, Govindaraju Archunan, Parasuraman Padmanabhan, Balázs Gulyás |
| Source: | Frontiers in Cellular and Infection Microbiology, Vol 12 (2022) |
| Publisher Information: | Frontiers Media S.A., 2022. |
| Publication Year: | 2022 |
| Collection: | LCC:Microbiology |
| Subject Terms: | Nocardiopsis exhalans, antimicrobial, antibiofilm, antioxidant, anticancer, acute toxicity, Microbiology, QR1-502 |
| More Details: | The present study aimed to isolate and identify potential drugs from marine actinomycete Nocardiopsis exhalans and screen them for biomedical applications. The cell-free culture of N. exhalans was extracted with ethyl acetate and the solvent extract showed six fractions in thin-layer chromatography. The fractions were subjected to column chromatography for purification and evaluated for activity against human clinical pathogens. Fraction 4 showed significant activity and was identified as N-(2-hydroxyphenyl)-2-phenazinamine (NHP) using spectral analyses. Further, NHP showed excellent biofilm inhibitory activity against human clinical pathogens Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The in vitro antioxidant activity confirmed that NHP is scavenging the oxidative stress-enhancing molecules. The anti-proliferative activity of NHP against human breast cancer cells showed significant activity at 300 µg/ml and less cytotoxic activity against normal cells. Additionally, the toxicity assessment against zebrafish revealed that NHP does not cause any toxicity in the important organs. The results highlight N. exhalans as a promising candidate for the development of antibiotics with potential therapeutic applications. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2235-2988 |
| Relation: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.794338/full; https://doaj.org/toc/2235-2988 |
| DOI: | 10.3389/fcimb.2022.794338 |
| Access URL: | https://doaj.org/article/ca1da715b7ca48eb86042c5d18176c39 |
| Accession Number: | edsdoj.1da715b7ca48eb86042c5d18176c39 |
| Database: | Directory of Open Access Journals |
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| Items | – Name: Title Label: Title Group: Ti Data: Structural Characterization, Antimicrobial, Antibiofilm, Antioxidant, Anticancer and Acute Toxicity Properties of N-(2-hydroxyphenyl)-2-phenazinamine From Nocardiopsis exhalans (KP149558) – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Vaikundamoorthy+Ramalingam%22">Vaikundamoorthy Ramalingam</searchLink><br /><searchLink fieldCode="AR" term="%22Rajendran+Rajaram%22">Rajendran Rajaram</searchLink><br /><searchLink fieldCode="AR" term="%22Govindaraju+Archunan%22">Govindaraju Archunan</searchLink><br /><searchLink fieldCode="AR" term="%22Parasuraman+Padmanabhan%22">Parasuraman Padmanabhan</searchLink><br /><searchLink fieldCode="AR" term="%22Balázs+Gulyás%22">Balázs Gulyás</searchLink> – Name: TitleSource Label: Source Group: Src Data: Frontiers in Cellular and Infection Microbiology, Vol 12 (2022) – Name: Publisher Label: Publisher Information Group: PubInfo Data: Frontiers Media S.A., 2022. – Name: DatePubCY Label: Publication Year Group: Date Data: 2022 – Name: Subset Label: Collection Group: HoldingsInfo Data: LCC:Microbiology – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Nocardiopsis+exhalans%22">Nocardiopsis exhalans</searchLink><br /><searchLink fieldCode="DE" term="%22antimicrobial%22">antimicrobial</searchLink><br /><searchLink fieldCode="DE" term="%22antibiofilm%22">antibiofilm</searchLink><br /><searchLink fieldCode="DE" term="%22antioxidant%22">antioxidant</searchLink><br /><searchLink fieldCode="DE" term="%22anticancer%22">anticancer</searchLink><br /><searchLink fieldCode="DE" term="%22acute+toxicity%22">acute toxicity</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiology%22">Microbiology</searchLink><br /><searchLink fieldCode="DE" term="%22QR1-502%22">QR1-502</searchLink> – Name: Abstract Label: Description Group: Ab Data: The present study aimed to isolate and identify potential drugs from marine actinomycete Nocardiopsis exhalans and screen them for biomedical applications. The cell-free culture of N. exhalans was extracted with ethyl acetate and the solvent extract showed six fractions in thin-layer chromatography. The fractions were subjected to column chromatography for purification and evaluated for activity against human clinical pathogens. Fraction 4 showed significant activity and was identified as N-(2-hydroxyphenyl)-2-phenazinamine (NHP) using spectral analyses. Further, NHP showed excellent biofilm inhibitory activity against human clinical pathogens Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The in vitro antioxidant activity confirmed that NHP is scavenging the oxidative stress-enhancing molecules. The anti-proliferative activity of NHP against human breast cancer cells showed significant activity at 300 µg/ml and less cytotoxic activity against normal cells. Additionally, the toxicity assessment against zebrafish revealed that NHP does not cause any toxicity in the important organs. The results highlight N. exhalans as a promising candidate for the development of antibiotics with potential therapeutic applications. – Name: TypeDocument Label: Document Type Group: TypDoc Data: article – Name: Format Label: File Description Group: SrcInfo Data: electronic resource – Name: Language Label: Language Group: Lang Data: English – Name: ISSN Label: ISSN Group: ISSN Data: 2235-2988 – Name: NoteTitleSource Label: Relation Group: SrcInfo Data: https://www.frontiersin.org/articles/10.3389/fcimb.2022.794338/full; https://doaj.org/toc/2235-2988 – Name: DOI Label: DOI Group: ID Data: 10.3389/fcimb.2022.794338 – Name: URL Label: Access URL Group: URL Data: <link linkTarget="URL" linkTerm="https://doaj.org/article/ca1da715b7ca48eb86042c5d18176c39" linkWindow="_blank">https://doaj.org/article/ca1da715b7ca48eb86042c5d18176c39</link> – Name: AN Label: Accession Number Group: ID Data: edsdoj.1da715b7ca48eb86042c5d18176c39 |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3389/fcimb.2022.794338 Languages: – Text: English Subjects: – SubjectFull: Nocardiopsis exhalans Type: general – SubjectFull: antimicrobial Type: general – SubjectFull: antibiofilm Type: general – SubjectFull: antioxidant Type: general – SubjectFull: anticancer Type: general – SubjectFull: acute toxicity Type: general – SubjectFull: Microbiology Type: general – SubjectFull: QR1-502 Type: general Titles: – TitleFull: Structural Characterization, Antimicrobial, Antibiofilm, Antioxidant, Anticancer and Acute Toxicity Properties of N-(2-hydroxyphenyl)-2-phenazinamine From Nocardiopsis exhalans (KP149558) Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Vaikundamoorthy Ramalingam – PersonEntity: Name: NameFull: Rajendran Rajaram – PersonEntity: Name: NameFull: Govindaraju Archunan – PersonEntity: Name: NameFull: Parasuraman Padmanabhan – PersonEntity: Name: NameFull: Balázs Gulyás IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 05 Type: published Y: 2022 Identifiers: – Type: issn-print Value: 22352988 Numbering: – Type: volume Value: 12 Titles: – TitleFull: Frontiers in Cellular and Infection Microbiology Type: main |
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